Summary of Study ST000229

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000185. The data can be accessed directly via it's Project DOI: 10.21228/M8BS32 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000229
Study TitleAnalysis of Dried Blood Spots by Paper Spray Ionization - MS
Study TypeAnalysis of Dried Blood Spots by Paper Spray Ionization - MS
Study SummaryConcentrations of cocaine (6, 5,3,2,1.5,1,0.75,0.5,0.25,0.125, 0.062, and 0.031 ppm) were spiked into whole human blood for PS-MS analysis. A 10 uL volume of blood/analyte mixture was allowed to dry on Whatman Grade 2 filter paper. A 1ppm concentration Cocaine-d3 was used as an internal standard. Parameters of the paper spray system were optimized, including spray voltage, capillary temperature and solvent. The internal standard was added on top of the dried blood spot sample. Wide isolation tandem MS was used to generate a calibration curve.
Institute
University of Florida
DepartmentChemistry
LaboratoryChemistry Lab Building
Last NameDhummakupt
First NameElizabeth
AddressR3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245
Emailemuffly@ufl.edu
Phone352-392-0515
Submit Date2015-07-16
Num Groups11
Total Subjects154
Study Comments11 concentrations were run as 7 samples/concentration for 2 days
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Uploaded File Size64 MB
Analysis Type DetailMS
Release Date2017-10-03
Release Version1
Elizabeth Dhummakupt Elizabeth Dhummakupt
https://dx.doi.org/10.21228/M8BS32
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cocaine concentration (ppb)
SA0108381ppmCoc_1ppmCocd3_intensity_AVG1000
SA010834125ppbCoc_1ppmCocd3_intensity_AVG125
SA0108391.5ppmCoc_1ppmCocd3_intensity_AVG1500
SA0108402ppmCoc_1ppmCocd3_intensity_AVG2000
SA010835250ppbCoc_1ppmCocd3_intensity_AVG250
SA0108413ppmCoc_1ppmCocd3_intensity_AVG3000
SA01083231ppbCoc_1ppmCocd3_intensity_AVG31
SA010836500ppbCoc_1ppmCocd3_intensity_AVG500
SA0108425ppmCoc_1ppmCocd3_intensity_AVG5000
SA0108436ppmCoc_1ppmCocd3_intensity_AVG6000
SA01083362ppbCoc_1ppmCocd3_intensity_AVG62
SA010837750ppbCoc_1ppmCocd3_intensity_AVG750
Showing results 1 to 12 of 12
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