Summary of Study ST000262
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000187. The data can be accessed directly via it's Project DOI: 10.21228/M8388M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000262 |
Study Title | Activity-Based Metabolic Profiling of M. tuberculosis H37Rv Rv1713 |
Study Type | timecourse |
Study Summary | These experiments involve incubating various proteins of unknown function in the M. tuberculosis genome with whole-cell lysate and assaying changes in metabolite levels over time (e.g. 0, 15, 30min) to try to infer what reaction they might catalyze. |
Institute | Weill Cornell Medicine |
Department | Microbiology and Immunology |
Last Name | Rhee |
First Name | Kyu |
Address | Department of Microbiology and Immunology, Weill Cornell Medical College, 1300 York Avenue, New York, NY |
kyr9001@med.cornell.edu | |
Submit Date | 2015-11-13 |
Num Groups | 3 replicates X 3 timepoints X with/without GTP |
Study Comments | The timepoints in the datafiles refer the amount of time (in minutes) that each protein was incubated with lysate.The units for 'mzmed' are mass/charge. The units for 'rtmed' are seconds (retention time). The units for all the other data columns in the spreadsheets are arbitrary; they are integrated peak counts. In this study, Rv1713 was incubated with lysate with and without 1mM GTP added as a co-factor. |
Raw Data Available | No |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 2 |
Release Comments | Updated study design factors |
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Factors:
Subject type: Bacterial cells; Subject species: Mycobacterium tuberculosis H37Rv (Factor headings shown in green)
mb_sample_id | local_sample_id | timepoint (min) | co-factor added to lysate |
---|---|---|---|
SA012013 | 1mMGTP1713_A_0 | 0 | 1mM GTP |
SA012019 | 1mMGTP1713_B_0 | 0 | 1mM GTP |
SA012025 | 1mMGTP1713_C_0 | 0 | 1mM GTP |
SA012012 | 1713_A_0 | 0 | None |
SA012018 | 1713_B_0 | 0 | None |
SA012024 | 1713_C_0 | 0 | None |
SA012015 | 1mMGTP1713_A_15 | 15 | 1mM GTP |
SA012021 | 1mMGTP1713_B_15 | 15 | 1mM GTP |
SA012027 | 1mMGTP1713_C_15 | 15 | 1mM GTP |
SA012014 | 1713_A_15 | 15 | None |
SA012020 | 1713_B_15 | 15 | None |
SA012026 | 1713_C_15 | 15 | None |
SA012017 | 1mMGTP1713_A_30 | 30 | 1mM GTP |
SA012023 | 1mMGTP1713_B_30 | 30 | 1mM GTP |
SA012029 | 1mMGTP1713_C_30 | 30 | 1mM GTP |
SA012016 | 1713_A_30 | 30 | None |
SA012022 | 1713_B_30 | 30 | None |
SA012028 | 1713_C_30 | 30 | None |
Showing results 1 to 18 of 18 |