Summary of Study ST002599

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST002599
Study Title	MoTrPAC: Endurance exercise training study in young adult rats, Tissue:Heart - Targeted Ethanolamides
Study Summary	The goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute	Emory University
Department	Biochemistry
Laboratory	Integrated Lipidomics and Metabolomics Core
Last Name	Ortlund
First Name	Eric
Address	1510 Clifton Rd. NE, Room G235, Atlanta, GA 30322
Email	eortlun@emory.edu
Phone	(404) 727-5014
Submit Date	2023-04-28
Raw Data Available	Yes
Raw Data File Type(s)	TBD
Analysis Type Detail	LC-MS
Release Date	2023-10-06
Release Version	1

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id	local_sample_id	Group	Timepoint	Sex
SA259925	90252015809	Control	8 weeks of training or control time	Female
SA259926	90266015809	Control	8 weeks of training or control time	Female
SA259927	90265015809	Control	8 weeks of training or control time	Female
SA259928	90248015809	Control	8 weeks of training or control time	Female
SA259929	90245015809	Control	8 weeks of training or control time	Female
SA259930	90237015809	Control	8 weeks of training or control time	Male
SA259931	90217015809	Control	8 weeks of training or control time	Male
SA259932	90239015809	Control	8 weeks of training or control time	Male
SA259933	90232015809	Control	8 weeks of training or control time	Male
SA259934	90229015809	Control	8 weeks of training or control time	Male
SA259935	QC_blank_69	QC-Blank	0 hour	-
SA259936	QC_blank_68	QC-Blank	0 hour	-
SA259937	QC_blank_65	QC-Blank	0 hour	-
SA259938	QC_blank_89	QC-Blank	0 hour	-
SA259939	QC_blank_4	QC-Blank	0 hour	-
SA259940	QC_blank_78	QC-Blank	0 hour	-
SA259941	QC_blank_90	QC-Blank	0 hour	-
SA259942	QC_blank_79	QC-Blank	0 hour	-
SA259943	QC_blank_80	QC-Blank	0 hour	-
SA259944	QC_Standard_71	QC-ExternalStandard	0 hour	-
SA259945	QC_Standard_81	QC-ExternalStandard	0 hour	-
SA259946	QC_Standard_70	QC-ExternalStandard	0 hour	-
SA259947	QC_Standard_72	QC-ExternalStandard	0 hour	-
SA259948	QC_Standard_77	QC-ExternalStandard	0 hour	-
SA259949	QC_Standard_82	QC-ExternalStandard	0 hour	-
SA259950	QC_Standard_73	QC-ExternalStandard	0 hour	-
SA259951	QC_Standard_88	QC-ExternalStandard	0 hour	-
SA259952	QC_Standard_75	QC-ExternalStandard	0 hour	-
SA259953	QC_Standard_76	QC-ExternalStandard	0 hour	-
SA259954	QC_Standard_87	QC-ExternalStandard	0 hour	-
SA259955	QC_Standard_74	QC-ExternalStandard	0 hour	-
SA259956	QC_Standard_85	QC-ExternalStandard	0 hour	-
SA259957	QC_Standard_86	QC-ExternalStandard	0 hour	-
SA259958	QC_Standard_83	QC-ExternalStandard	0 hour	-
SA259959	QC_Standard_84	QC-ExternalStandard	0 hour	-
SA259960	QC_62	QC-Pooled	0 hour	-
SA259961	QC_29	QC-Pooled	0 hour	-
SA259962	QC_7	QC-Pooled	0 hour	-
SA259963	QC_40	QC-Pooled	0 hour	-
SA259964	QC_51	QC-Pooled	0 hour	-
SA259965	QC_18	QC-Pooled	0 hour	-
SA259966	QC_67	QC-Pooled	0 hour	-
SA259967	QC_prerun_1	QC-PreRun	0 hour	-
SA259968	QC_prerun_2	QC-PreRun	0 hour	-
SA259969	QC_prerun_3	QC-PreRun	0 hour	-
SA259970	G_QC_1_5	QC-Reference	0 hour	-
SA259971	G_QC_1_63	QC-Reference	0 hour	-
SA259972	QC_SRM	QC-Reference	0 hour	-
SA259973	G_QC_2_64	QC-Reference	0 hour	-
SA259974	G_QC_2_6	QC-Reference	0 hour	-
SA259975	90559015809	Training	1 week of training or control time	Female
SA259976	90560015809	Training	1 week of training or control time	Female
SA259977	90571015809	Training	1 week of training or control time	Female
SA259978	90567015809	Training	1 week of training or control time	Female
SA259979	90564015809	Training	1 week of training or control time	Female
SA259980	90423015809	Training	1 week of training or control time	Male
SA259981	90430015809	Training	1 week of training or control time	Male
SA259982	90426015809	Training	1 week of training or control time	Male
SA259983	90422015809	Training	1 week of training or control time	Male
SA259984	90421015809	Training	1 week of training or control time	Male
SA259985	90587015809	Training	2 weeks of training	Female
SA259986	90576015809	Training	2 weeks of training	Female
SA259987	90578015809	Training	2 weeks of training	Female
SA259988	90581015809	Training	2 weeks of training	Female
SA259989	90585015809	Training	2 weeks of training	Female
SA259990	90449015809	Training	2 weeks of training	Male
SA259991	90444015809	Training	2 weeks of training	Male
SA259992	90450015809	Training	2 weeks of training	Male
SA259993	90441015809	Training	2 weeks of training	Male
SA259994	90439015809	Training	2 weeks of training	Male
SA259995	90420015809	Training	4 weeks of training	Female
SA259996	90410015809	Training	4 weeks of training	Female
SA259997	90406015809	Training	4 weeks of training	Female
SA259998	90416015809	Training	4 weeks of training	Female
SA259999	90412015809	Training	4 weeks of training	Female
SA260000	90289015809	Training	4 weeks of training	Male
SA260001	90280015809	Training	4 weeks of training	Male
SA260002	90283015809	Training	4 weeks of training	Male
SA260003	90281015809	Training	4 weeks of training	Male
SA260004	90292015809	Training	4 weeks of training	Male
SA260005	90251015809	Training	8 weeks of training or control time	Female
SA260006	90258015809	Training	8 weeks of training or control time	Female
SA260007	90267015809	Training	8 weeks of training or control time	Female
SA260008	90254015809	Training	8 weeks of training or control time	Female
SA260009	90259015809	Training	8 weeks of training or control time	Female
SA260010	90223015809	Training	8 weeks of training or control time	Male
SA260011	90227015809	Training	8 weeks of training or control time	Male
SA260012	90222015809	Training	8 weeks of training or control time	Male
SA260013	90218015809	Training	8 weeks of training or control time	Male
SA260014	90225015809	Training	8 weeks of training or control time	Male

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