Summary of Study ST002613

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001020. The data can be accessed directly via it's Project DOI: 10.21228/M8V97D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002613
Study TitleMoTrPAC: Endurance exercise training study in young adult rats, Tissue:Liver - Targeted Ethanolamides
Study SummaryThe goal of the endurance exercise training study in young adult rats (internal code: PASS1B-06) was to perform exercise training studies in adult (6 month) F344 rats, and from these rats collect as many tissues as feasible in order to provide high quality samples for detailed analysis by chemical analysis sites. Tissues were collected from 10-12 rats sedentary control rats concurrent with the collection of the 8-week training groups. The 8-week training group and controls were from the same cohort and same age at euthanasia (either 8). For the older age group, an additional set of controls (n=5-6) were collected with the 1-2 week training group. Rats were either sedentary or underwent an exercise training program. Rats were exercised on the rodent treadmill 5 days per week using a progressive training protocol designed to exercise the rats at approximately 70% of VO2max as outlined in the Table on the next page. Training was performed no earlier than 10:00 am and no later than 5:00 pm over 5 consecutive days per week. Training was initiated with the treadmill set at 70% of VO2 max (see tables) and 5 degrees grade for 20 minutes. The duration of exercise was increased by one minute each day until day 31 of training (start of week 7), when a duration of 50 min was reached. Speed and grade of each training session increased in larger increments due to treadmill parameters. The highest intensity and duration of training began on day 31. This intensity was maintained for the final 10 days of the protocol to ensure steady state had been achieved. If any rats were unable to perform at least 4 days of training per week they were removed from the study and euthanized. It is important to note that the starting treadmill speed varied depending on the sex and age of the rat. The initial and maximum speeds were based on VO2max measurements obtained during the pre-training testing of the compliant rats. Rats assigned to the control group followed a schedule similar to the training group. They were placed in one lane on the treadmill for 15 minutes/day, 5 days per week. The treadmill was set at 0 m/min at an incline that corresponded to the incline being used by the training group.
Institute
Emory University
DepartmentBiochemistry
LaboratoryIntegrated Lipidomics and Metabolomics Core
Last NameOrtlund
First NameEric
Address1510 Clifton Rd. NE, Room G235, Atlanta, GA 30322
Emaileortlun@emory.edu
Phone(404) 727-5014
Submit Date2023-04-28
Raw Data AvailableYes
Raw Data File Type(s)TBD
Analysis Type DetailLC-MS
Release Date2023-10-06
Release Version1
Eric Ortlund Eric Ortlund
https://dx.doi.org/10.21228/M8V97D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Timepoint Sex
SA26083290266016810Control 8 weeks of training or control time Female
SA26083390252016810Control 8 weeks of training or control time Female
SA26083490248016810Control 8 weeks of training or control time Female
SA26083590265016810Control 8 weeks of training or control time Female
SA26083690245016810Control 8 weeks of training or control time Female
SA26083790229016810Control 8 weeks of training or control time Male
SA26083890237016810Control 8 weeks of training or control time Male
SA26083990217016810Control 8 weeks of training or control time Male
SA26084090239016810Control 8 weeks of training or control time Male
SA26084190232016810Control 8 weeks of training or control time Male
SA260842QC_blank_79QC-Blank 0 hour -
SA260843QC_blank_69QC-Blank 0 hour -
SA260844QC_blank_4QC-Blank 0 hour -
SA260845QC_blank_78QC-Blank 0 hour -
SA260846QC_blank_68QC-Blank 0 hour -
SA260847QC_blank_66QC-Blank 0 hour -
SA260848QC_Standard_71QC-ExternalStandard 0 hour -
SA260849QC_Standard_75QC-ExternalStandard 0 hour -
SA260850QC_Standard_76QC-ExternalStandard 0 hour -
SA260851QC_Standard_77QC-ExternalStandard 0 hour -
SA260852QC_Standard_74QC-ExternalStandard 0 hour -
SA260853QC_Standard_73QC-ExternalStandard 0 hour -
SA260854QC_Standard_70QC-ExternalStandard 0 hour -
SA260855QC_Standard_72QC-ExternalStandard 0 hour -
SA260856QC_62QC-Pooled 0 hour -
SA260857QC_51QC-Pooled 0 hour -
SA260858QC_67QC-Pooled 0 hour -
SA260859QC_40QC-Pooled 0 hour -
SA260860QC_18QC-Pooled 0 hour -
SA260861QC_7QC-Pooled 0 hour -
SA260862QC_29QC-Pooled 0 hour -
SA260863QC_prerun_2QC-PreRun 0 hour -
SA260864QC_prerun_1QC-PreRun 0 hour -
SA260865QC_prerun_3QC-PreRun 0 hour -
SA260866QC_SRMQC-Reference 0 hour -
SA260867G_QC_1_63QC-Reference 0 hour -
SA260868G_QC_1_5QC-Reference 0 hour -
SA260869G_QC_2_6QC-Reference 0 hour -
SA260870G_QC_2_64QC-Reference 0 hour -
SA26087190560016810Training 1 week of training or control time Female
SA26087290559016810Training 1 week of training or control time Female
SA26087390571016810Training 1 week of training or control time Female
SA26087490564016810Training 1 week of training or control time Female
SA26087590567016810Training 1 week of training or control time Female
SA26087690422016810Training 1 week of training or control time Male
SA26087790430016810Training 1 week of training or control time Male
SA26087890423016810Training 1 week of training or control time Male
SA26087990421016810Training 1 week of training or control time Male
SA26088090426016810Training 1 week of training or control time Male
SA26088190578016810Training 2 weeks of training Female
SA26088290576016810Training 2 weeks of training Female
SA26088390581016810Training 2 weeks of training Female
SA26088490585016810Training 2 weeks of training Female
SA26088590587016810Training 2 weeks of training Female
SA26088690450016810Training 2 weeks of training Male
SA26088790441016810Training 2 weeks of training Male
SA26088890444016810Training 2 weeks of training Male
SA26088990449016810Training 2 weeks of training Male
SA26089090439016810Training 2 weeks of training Male
SA26089190412016810Training 4 weeks of training Female
SA26089290406016810Training 4 weeks of training Female
SA26089390410016810Training 4 weeks of training Female
SA26089490416016810Training 4 weeks of training Female
SA26089590420016810Training 4 weeks of training Female
SA26089690280016810Training 4 weeks of training Male
SA26089790289016810Training 4 weeks of training Male
SA26089890283016810Training 4 weeks of training Male
SA26089990292016810Training 4 weeks of training Male
SA26090090281016810Training 4 weeks of training Male
SA26090190254016810Training 8 weeks of training or control time Female
SA26090290251016810Training 8 weeks of training or control time Female
SA26090390259016810Training 8 weeks of training or control time Female
SA26090490258016810Training 8 weeks of training or control time Female
SA26090590267016810Training 8 weeks of training or control time Female
SA26090690222016810Training 8 weeks of training or control time Male
SA26090790227016810Training 8 weeks of training or control time Male
SA26090890218016810Training 8 weeks of training or control time Male
SA26090990225016810Training 8 weeks of training or control time Male
SA26091090223016810Training 8 weeks of training or control time Male
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