Summary of Study ST002810

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001757. The data can be accessed directly via it's Project DOI: 10.21228/M8M13Z This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002810
Study TitleGAS2 encodes a 2-oxoglutarate dependent dioxygenase involved in ABA catabolism
Study SummaryLiu et al. [1] recently reported the characterization of Arabidopsis thaliana GAS2 (Gain of Function in ABA-modulated Seed Germination 2), which was described as an enzyme that catalyzes the stereospecific hydration of GA12 to produce GA12 16, 17-dihydro-16α-ol (DHGA12). A second paper describes the conversion of GA12 to an unidentified product by GAS2 and also reports that this enzyme does not convert ABA [2]. However, as previously reported [3], we did not find any conversion of [17-14C]-labeled or [1-,7-,12-,18-14C4]-labeled GA12 by GAS2. Instead, we present here data showing that the recombinant GAS2 enzyme is capable of catabolising abscisic acid (ABA) to phaseic acid (PA) and further to a second product, putative 8’-carboxy-ABA (compound A; Fig. 1a) [4]. References: [1] Liu, H. et al. Biosynthesis of DHGA12 and its roles in Arabidopsis seedling establishment. Nat. Commun. 10, 1768 (2019). [2] Xiong, W. et al. The dioxygenase GIM2 functions in seed germination by altering gibberellin production in Arabidopsis. J. Integr. Plant Biol. 60, 276-291 (2018). [3] Lange, T. & Pimenta Lange, M. J. The Multifunctional Dioxygenases of Gibberellin Synthesis. Plant Cell Physiol. 61, 1869-1879 (2020). [4] Lange, T., Atiq, N., Pimenta Lange. GAS2 encodes a 2-oxoglutarate dependent dioxygenase involved in ABA catabolism. bioRxiv, doi: 10.1101/2022.11.16.516706 (2022).
Institute
Technische Universität Braunschweig
DepartmentBiochemie und Physiology der Pflanzen
LaboratoryAG Lange
Last NameLange
First NameTheo
AddressMendelssohnstr. 4
Emailtheo.lange@tu-bs.de
Phone00495313915880
Submit Date2023-08-01
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailGC-MS
Release Date2023-10-11
Release Version1
Theo Lange Theo Lange
https://dx.doi.org/10.21228/M8M13Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Plant; Subject species: Arabidopsis thaliana (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA301609551_1020ox1
SA301610552_1120ox1
SA301611550_0920ox1
SA301612559_03ABA
SA301642539_06d3-PA
SA301643868_08d6-ABA
SA301613266_01DHGA12
SA301614920_10GAS2
SA301615967_01GAS2
SA301616907_09GAS2
SA301617969_02GAS2
SA301618748_05GAS2
SA301619747_04GAS2
SA301620970_03GAS2
SA301621990_02GAS2
SA301622403_02GAS2
SA301623976_07GAS2
SA301624975_06GAS2
SA301625972_04GAS2
SA301626974_05GAS2
SA301627746_03GAS2
SA301628691_02GAS2
SA301629543_08GAS2
SA301630481_03GAS2
SA301631542_07GAS2
SA301632486_06GAS2
SA301633485_05GAS2
SA301634482_04GAS2
SA301635480_02GAS2
SA301636583_04GAS2
SA301637585_05GAS2
SA301638479_01GAS2
SA301639556_02GAS2
SA301640558_07GAS2
SA301641538_01PA
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