Summary of Study ST002990

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001773. The data can be accessed directly via it's Project DOI: 10.21228/M8J420 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002990
Study TitleFolate depletion time-course in K562 cells with analysis for porphyrin metabolites
Study SummaryCulture of K562 cells in RPMI media containing 100 nM folic acid for 0, 1, 2, 4, 6, or 8 days followed my LC-MS targeting porphyrin metabolites. This is a reverse timecourse where all samples are harvested on the same day. Day 0 in 100 nM folic acid indicates 8 days culture in 2,000 nM folic acid.
Institute
Boston Children's Hospital, Harvard Medical School
Departmentpathology
LaboratoryKanarek Lab
Last NameKanarek
First NameNaama
AddressEnders 1116.2, 300 Longwood Ave, Boston, MA 02115
Emailnaama.kanarek@childrens.harvard.edu
Phone6173557433
Submit Date2023-08-23
Num Groups5
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-02-13
Release Version1
Naama Kanarek Naama Kanarek
https://dx.doi.org/10.21228/M8J420
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id 100nM_Folic_Acid_Growth
SA325766K562_100nM_Timecourse_Day0_Porphyrin_1Day0
SA325767K562_100nM_Timecourse_Day0_Porphyrin_4Day0
SA325768K562_100nM_Timecourse_Day0_Porphyrin_3Day0
SA325769K562_100nM_Timecourse_Day0_Porphyrin_2Day0
SA325770K562_100nM_Timecourse_Day1_Porphyrin_4Day1
SA325771K562_100nM_Timecourse_Day1_Porphyrin_1Day1
SA325772K562_100nM_Timecourse_Day1_Porphyrin_3Day1
SA325773K562_100nM_Timecourse_Day1_Porphyrin_2Day1
SA325774K562_100nM_Timecourse_Day2_Porphyrin_4Day2
SA325775K562_100nM_Timecourse_Day2_Porphyrin_3Day2
SA325776K562_100nM_Timecourse_Day2_Porphyrin_2Day2
SA325777K562_100nM_Timecourse_Day2_Porphyrin_1Day2
SA325778K562_100nM_Timecourse_Day4_Porphyrin_3Day4
SA325779K562_100nM_Timecourse_Day4_Porphyrin_4Day4
SA325780K562_100nM_Timecourse_Day4_Porphyrin_2Day4
SA325781K562_100nM_Timecourse_Day4_Porphyrin_1Day4
SA325782K562_100nM_Timecourse_Day6_Porphyrin_4Day6
SA325783K562_100nM_Timecourse_Day6_Porphyrin_2Day6
SA325784K562_100nM_Timecourse_Day6_Porphyrin_1Day6
SA325785K562_100nM_Timecourse_Day6_Porphyrin_3Day6
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