Summary of Study ST003149
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001958. The data can be accessed directly via it's Project DOI: 10.21228/M8MX5P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003149 |
| Study Title | Impact of early-life exposure to a potent aryl hydrocarbon receptor ligand on gut microbiota and host glucose homeostasis in C57BL/6J male mice (Part I) |
| Study Summary | This study aimed to explore the association between early-life exposure to a potent aryl hydrocarbon receptor (AHR) agonist and persistent disruptions in the microbiota, leading to impaired metabolic homeostasis later in life. This study utilized metagenomics, NMR- and mass spectrometry-based metabolomics, and biochemical assays to analyze the gut microbiome composition and function, as well as the physiological and metabolic effects of early-life exposure to 2,3,7,8-tetrachlorodibenzofuran (TCDF) in conventional, germ-free (GF), and Ahr-null mice. The impact of TCDF on Akkermansia muciniphila (A. muciniphila) in vitro was assessed using optical density (OD 600), flow cytometry, transcriptomics, and mass spectrometry-based metabolomics. TCDF-exposed mice exhibited disruption in the gut microbiome community structure and function, characterized by lower abundances of A. muciniphila, lower levels of cecal short chain fatty acids (SCFAs) and indole-3-lactic acid (ILA), and a reduction in gut hormones GLP-1 and PYY. Importantly, microbial and metabolic phenotypes associated with early-life POP exposure were transferable to GF recipients in the absence of POP carry-over. In addition, AHR-independent interactions between POPs and the microbiota were observed, significantly affected the growth, physiology, gene expression, and metabolic activity of A. muciniphila, resulting in suppressed activity along the ILA pathway. |
| Institute | Pennsylvania State University |
| Department | Department of Veterinary and Biomedical Sciences |
| Last Name | Koo |
| First Name | Imhoi |
| Address | 307B Life Science Building |
| iuk41@psu.edu | |
| Phone | 8148107425 |
| Submit Date | 2024-03-28 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2024-04-30 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source |
|---|---|---|
| SA340775 | GF.cecal.content_Akkermansia__8 | cecal content | Genotype:GF C57BL/6J mice | Treatment:ABX water |
| SA340776 | GF.cecal.content_Akkermansia__9 | cecal content | Genotype:GF C57BL/6J mice | Treatment:ABX water |
| SA340777 | GF.cecal.content_Akkermansia__10 | cecal content | Genotype:GF C57BL/6J mice | Treatment:ABX water |
| SA340778 | GF.cecal.content_Akkermansia__3 | cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia |
| SA340779 | GF.cecal.content_Akkermansia__4 | cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia |
| SA340780 | GF.cecal.content_Akkermansia__5 | cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia |
| SA340781 | GF.cecal.content_Akkermansia__1 | cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia |
| SA340782 | GF.cecal.content_Akkermansia__2 | cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia |
| SA340783 | GF.cecal.content_Akkermansia__11 | cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia +ABX water |
| SA340784 | GF.cecal.content_Akkermansia__12 | cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia +ABX water |
| SA340785 | GF.cecal.content_Akkermansia__6 | cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia +ABX water |
| SA340786 | GF.cecal.content_Akkermansia__7 | cecal content | Genotype:GF C57BL/6J mice | Treatment:Akkermansia +ABX water |
| SA340787 | GF.cecal.content_cecal.bacteria__9 | cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group |
| SA340788 | GF.cecal.content_cecal.bacteria__6 | cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group |
| SA340789 | GF.cecal.content_cecal.bacteria__8 | cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group |
| SA340790 | GF.cecal.content_cecal.bacteria__7 | cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group |
| SA340791 | GF.cecal.content_cecal.bacteria__3 | cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group |
| SA340792 | GF.cecal.content_cecal.bacteria__1 | cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group |
| SA340793 | GF.cecal.content_cecal.bacteria__4 | cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group |
| SA340794 | GF.cecal.content_cecal.bacteria__2 | cecal content | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group |
| SA340795 | cecal.content.with.TCDF_long__10 | cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340796 | cecal.content.with.TCDF_long__9 | cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340797 | cecal.content.with.TCDF_long__11 | cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340798 | cecal.content.with.TCDF_long__12 | cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340799 | cecal.content.with.TCDF_long__7 | cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340800 | cecal.content.with.TCDF_short__12 | cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340801 | cecal.content.with.TCDF_short__11 | cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340802 | cecal.content.with.TCDF_short__7 | cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340803 | cecal.content.with.TCDF_short__8 | cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340804 | cecal.content.with.TCDF_short__9 | cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340805 | cecal.content.with.TCDF_short__10 | cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340806 | cecal.content.with.TCDF_long__8 | cecal content | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340807 | cecal.content.with.TCDF_long__2 | cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340808 | cecal.content.with.TCDF_long__3 | cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340809 | cecal.content.with.TCDF_long__4 | cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340810 | cecal.content.with.TCDF_long__6 | cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340811 | cecal.content.with.TCDF_short__2 | cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340812 | cecal.content.with.TCDF_short__5 | cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340813 | cecal.content.with.TCDF_short__6 | cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340814 | cecal.content.with.TCDF_short__4 | cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340815 | cecal.content.with.TCDF_short__3 | cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340816 | cecal.content.with.TCDF_long__1 | cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340817 | cecal.content.with.TCDF_short__1 | cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340818 | cecal.content.with.TCDF_long__5 | cecal content | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340819 | AHR.KO.liver.lipid.with.TCDF__5 | liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:TCDF |
| SA340820 | AHR.KO.liver.lipid.with.TCDF__6 | liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:TCDF |
| SA340821 | AHR.KO.liver.lipid.with.TCDF__8 | liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:TCDF |
| SA340822 | AHR.KO.liver.lipid.with.TCDF__4 | liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:TCDF |
| SA340823 | AHR.KO.liver.lipid.with.TCDF__7 | liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:vehicle |
| SA340824 | AHR.KO.liver.lipid.with.TCDF__3 | liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:vehicle |
| SA340825 | AHR.KO.liver.lipid.with.TCDF__2 | liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:vehicle |
| SA340826 | AHR.KO.liver.lipid.with.TCDF__1 | liver | Genotype:Ahr-/- C57BL/6J mice | Treatment:vehicle |
| SA340831 | GF.liver.lipid._cecal.bacteria__6 | liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group |
| SA340832 | GF.liver.lipid._cecal.bacteria__9 | liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group |
| SA340833 | GF.liver.lipid._cecal.bacteria__10 | liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group |
| SA340834 | GF.liver.lipid._cecal.bacteria__8 | liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group |
| SA340835 | GF.liver.lipid._cecal.bacteria__7 | liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from TCDF group |
| SA340836 | GF.liver.lipid._cecal.bacteria__1 | liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group |
| SA340837 | GF.liver.lipid._cecal.bacteria__5 | liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group |
| SA340838 | GF.liver.lipid._cecal.bacteria__4 | liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group |
| SA340839 | GF.liver.lipid._cecal.bacteria__3 | liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group |
| SA340840 | GF.liver.lipid._cecal.bacteria__2 | liver | Genotype:GF C57BL/6J mice | Treatment:cecal gavage from vehicle group |
| SA340827 | GF.liver.lipid.with.TCDF__6 | liver | Genotype:GF C57BL/6J mice | Treatment:TCDF |
| SA340828 | GF.liver.lipid.with.TCDF__7 | liver | Genotype:GF C57BL/6J mice | Treatment:TCDF |
| SA340829 | GF.liver.lipid.with.TCDF__5 | liver | Genotype:GF C57BL/6J mice | Treatment:TCDF |
| SA340830 | GF.liver.lipid.with.TCDF__8 | liver | Genotype:GF C57BL/6J mice | Treatment:TCDF |
| SA340841 | GF.liver.lipid.with.TCDF__3 | liver | Genotype:GF C57BL/6J mice | Treatment:vehicle |
| SA340842 | GF.liver.lipid.with.TCDF__2 | liver | Genotype:GF C57BL/6J mice | Treatment:vehicle |
| SA340843 | GF.liver.lipid.with.TCDF__4 | liver | Genotype:GF C57BL/6J mice | Treatment:vehicle |
| SA340844 | GF.liver.lipid.with.TCDF__1 | liver | Genotype:GF C57BL/6J mice | Treatment:vehicle |
| SA340845 | liver.lipid.with.TCDF_short__111 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340846 | liver.lipid.with.TCDF_short__110 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340847 | liver.lipid.with.TCDF_short__109 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340848 | liver.lipid.with.TCDF_short__107 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340849 | liver.lipid.with.TCDF_short__108 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340850 | liver.lipid.with.TCDF_short__112 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340851 | liver.with.TCDF_.long__12 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340852 | liver.with.TCDF_.long__7 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340853 | liver.with.TCDF_short__7 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340854 | liver.with.TCDF_.long__9 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340855 | liver.with.TCDF_.long__10 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340856 | liver.with.TCDF_.long__11 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340857 | liver.with.TCDF_short__12 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340858 | liver.lipid.with.TCDF_long__207 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340859 | liver.lipid.with.TCDF_long__208 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340860 | liver.with.TCDF_short__9 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340861 | liver.with.TCDF_short__10 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340862 | liver.with.TCDF_.long__8 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340863 | liver.with.TCDF_short__11 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340864 | liver.lipid.with.TCDF_long__210 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340865 | liver.lipid.with.TCDF_long__209 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340866 | liver.with.TCDF_short__8 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340867 | liver.lipid.with.TCDF_long__212 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340868 | liver.lipid.with.TCDF_long__211 | liver | Genotype:WT C57BL/6J mice | Treatment:TCDF |
| SA340869 | liver.with.TCDF_short__4 | liver | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340870 | liver.with.TCDF_short__5 | liver | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340871 | liver.with.TCDF_short__3 | liver | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340872 | liver.with.TCDF_short__2 | liver | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340873 | liver.with.TCDF_short__6 | liver | Genotype:WT C57BL/6J mice | Treatment:vehicle |
| SA340874 | liver.with.TCDF_short__1 | liver | Genotype:WT C57BL/6J mice | Treatment:vehicle |
