Summary of Study ST003182
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001981. The data can be accessed directly via it's Project DOI: 10.21228/M8NQ82 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003182 |
| Study Title | METTL3-mediated chromatin contacts promote stress granule phase separation through metabolic reprogramming during senescence |
| Study Summary | METTL3 is the catalytic subunit of the methyltransferase complex, which mediates m6A modification to regulate gene expression. In addition, METTL3 regulates transcription in an enzymatic activity-independent manner by driving changes in high-order chromatin structure. However, how these functions of MTC are coordinated remains unknown. Here we show that the methyltransferase complex coordinates its enzymatic activity-dependent and independent functions to regulate cellular senescence, a state of stable cell growth arrest. Specifically, METTL3-mediated chromatin loops induce Hexokinase 2 expression through the three-dimensional chromatin organization during senescence. Elevated Hexokinase 2 expression subsequently promotes liquid-liquid phase separation, manifesting as stress granule phase separation, by driving metabolic reprogramming. This correlates with an impairment of translation of cell-cycle related mRNAs harboring polymethylated m6A sites. In summary, our results report a coordination of m6A-dependent and -independent function of the methyltransferase complex in regulating senescence through phase separation driven by metabolic reprogramming. |
| Institute | University of Texas MD Anderson Cancer Center |
| Last Name | Zhang |
| First Name | Rugang |
| Address | 3SCR3.4121, 1901 East RD, Houston, TX, 77054 |
| rzhang11@mdanderson.org | |
| Phone | 832-748-6422 |
| Submit Date | 2024-04-29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-05-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment | Sample source |
|---|---|---|---|
| SA346713 | Pro_2 | Pro | lung embryonic fibroblasts |
| SA346714 | Pro_1 | Pro | lung embryonic fibroblasts |
| SA346715 | Pro_3 | Pro | lung embryonic fibroblasts |
| SA346725 | Sen_shctrl_1 | Sen_shctrl | lung embryonic fibroblasts |
| SA346726 | Sen_shctrl_3 | Sen_shctrl | lung embryonic fibroblasts |
| SA346727 | Sen_shctrl_2 | Sen_shctrl | lung embryonic fibroblasts |
| SA346716 | Sen_shHK2_MutHK2_3 | Sen_shHK2_MutHK2 | lung embryonic fibroblasts |
| SA346717 | Sen_shHK2_MutHK2_2 | Sen_shHK2_MutHK2 | lung embryonic fibroblasts |
| SA346718 | Sen_shHK2_MutHK2_1 | Sen_shHK2_MutHK2 | lung embryonic fibroblasts |
| SA346722 | Sen_shHK2_2 | Sen_shHK2 | lung embryonic fibroblasts |
| SA346723 | Sen_shHK2_3 | Sen_shHK2 | lung embryonic fibroblasts |
| SA346724 | Sen_shHK2_1 | Sen_shHK2 | lung embryonic fibroblasts |
| SA346719 | Sen_shHK2_WTHK2_3 | Sen_shHK2_WTHK2 | lung embryonic fibroblasts |
| SA346720 | Sen_shHK2_WTHK2_2 | Sen_shHK2_WTHK2 | lung embryonic fibroblasts |
| SA346721 | Sen_shHK2_WTHK2_1 | Sen_shHK2_WTHK2 | lung embryonic fibroblasts |
| Showing results 1 to 15 of 15 |
