Summary of Study ST001194

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000806. The data can be accessed directly via it's Project DOI: 10.21228/M8H390 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001194
Study TitleFlavonoid study of Ginkgo leaves facing to different elevation and plant age
Study SummaryGinkgo biloba leaves are always resources for flavonoids pharmaceutical industry. Thus, artificial planting and industrial harvesting become the vital aspect to get higher drug yields. In this research, we performed de novo transcriptome sequencing of Ginkgo leaves coupled with high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry analyses to obtain a comprehensive understanding of the influence of elevation and plant age on flavonoid synthesis. A total of 557,659,530 clean reads were assembled into 188,155 unigenes, of which 135,102 (71.80%) were successfully annotated in seven public databases. The differentially expressed genes analysis indicated DFR, LAR and ANR were significantly up-regulated with the increase of elevation in young Ginkgo trees leaves. With less strict saliency, the relative concentration of flavonoid derivatives with high parent ion signal intensity was likely to support this conclusion. Complex gene variations were observed with the plant age change. However, flavonoid derivatives analysis predicted the potential possibility that the rise of plant age is more likely to be detrimental to the biosynthesis of Ginkgo flavonoids in leaves. From the overall DEGs involved in flavonoid biosynthesis, DFRs seemed to show more considerable variability towards the variation of elevation and plant age. Furthermore, our research effectively expanded the functional genomic library of Ginkgo and provided a reference for artificial planting and industrial harvesting.
Institute
Central South University, China
Last NameZou
First NameKai
AddressCentral South University, 932 Lushan South Road, Yuelu District, Changsha City, Hunan Province
Emailzoukai3412085@hotmail.com
Phone+8615273119784
Submit Date2019-05-05
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2019-07-05
Release Version1
Kai Zou Kai Zou
https://dx.doi.org/10.21228/M8H390
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Plant; Subject species: Ginkgo Biloba (Factor headings shown in green)

mb_sample_id local_sample_id Elevation Relative Age
SA083085HO-2High Old
SA083086HO-1High Old
SA083087HO-3High Old
SA083088HY-3High Young
SA083089HY-1High Young
SA083090HY-2High Young
SA083091LO-3Low Old
SA083092LO-1Low Old
SA083093LO-2Low Old
SA083094LY-2Low Young
SA083095LY-1Low Young
SA083096LY-3Low Young
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