Summary of Study ST001786

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001135. The data can be accessed directly via it's Project DOI: 10.21228/M80D82 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001786
Study TitleMulti-omics analysis of glucose-mediated signaling by a moonlighting Gβ protein Asc1/RACK1
Study TypeUntargeted UPLC-MS Metabolomics Analysis
Study SummaryWhile much is known about glucose metabolism in yeast, less is known about the receptors and signaling pathways that indicate glucose availability. We obtained metabolic profiles for wildtype and 16 mutants affecting the yeast glucose sensing pathway, comparing 0.05% glucose vs 10 min after glucose addition to 2%. First, we determined that the G protein-coupled receptor (Gpr1/Gpa2) directs early events in glucose utilization while the transceptors (Snf3/Rgt2) regulate subsequent processes and downstream products of glucose metabolism. Whereas the large G protein transmits the signal from its cognate receptor, Ras2 (but not Ras1) integrates responses from both receptor pathways. Second, we determined the relative contributions of the G protein α (Gpa2) and β (Asc1) subunits to glucose-initiated processes. We determined that Gpa2 is primarily involved in regulating carbohydrate metabolism while Asc1 is primarily involved in amino acid metabolism. Both proteins are involved in regulating purine metabolism. Collectively, our analysis reveals the molecular basis for glucose detection and the earliest events of glucose-dependent signal transduction in yeast.
Institute
University of North Carolina at Chapel Hill
DepartmentNutrition
LaboratoryMetabolomics and Exposome Laboratory, Nutrition Research Institute, UNC Chapel Hill
Last NameSumner
First NameSusan
Address500 Laureate Way, Nutrition Research Institute, UNC Chapel Hill
Emailsusan_sumner@unc.edu
Phone(919) 622-4456
Submit Date2021-05-10
Num Groups28
Total Subjects192
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-06-11
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M80D82
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Yeast; Subject species: Saccharomyces cerevisiae (Factor headings shown in green)

mb_sample_id local_sample_id Genotype Condition
SA166255asc1_H5_Aasc1 H
SA166256asc1_H4_Aasc1 H
SA166257asc1_H3_Aasc1 H
SA166258asc1_H6_Aasc1 H
SA166259asc1_H1_Aasc1 H
SA166260asc1_L4_Aasc1 L
SA166261asc1_L3_Aasc1 L
SA166262asc1_L2_Aasc1 L
SA166263asc1_L1_Aasc1 L
SA166264asc1_L6_Aasc1 L
SA166265asc1_L5_Aasc1 L
SA166266gpa2_H5_Agpa2 H
SA166267gpa2_H6_Agpa2 H
SA166268gpa2_H4_Agpa2 H
SA166269gpa2_H3_Agpa2 H
SA166270gpa2_H2_Agpa2 H
SA166271gpa2_H1_Agpa2 H
SA166272gpa2_L5_Agpa2 L
SA166273gpa2_L6_Agpa2 L
SA166274gpa2_L4_Agpa2 L
SA166275gpa2_L2_Agpa2 L
SA166276gpa2_L1_Agpa2 L
SA166277gpa2_L3_Agpa2 L
SA166278gpr1_H5_Agpr1 H
SA166279gpr1_H4_Agpr1 H
SA166280gpr1_H6_Agpr1 H
SA166281gpr1_H1_Agpr1 H
SA166282gpr1_H3_Agpr1 H
SA166283gpr1_H2_Agpr1 H
SA166284gpr1_L4_Agpr1 L
SA166285gpr1_L6_Agpr1 L
SA166286gpr1_L5_Agpr1 L
SA166287gpr1_L3_Agpr1 L
SA166288gpr1_L2_Agpr1 L
SA166289gpr1_L1_Agpr1 L
SA166290pde1_H4_Bpde1 H
SA166291pde1_H1_Bpde1 H
SA166292pde1_H2_Bpde1 H
SA166293pde1_H3_Bpde1 H
SA166294pde1_H5_Bpde1 H
SA166295pde1_H6_Bpde1 H
SA166296pde1_L1_Bpde1 L
SA166297pde1_L2_Bpde1 L
SA166298pde1_L3_Bpde1 L
SA166299pde1_L4_Bpde1 L
SA166300pde1_L5_Bpde1 L
SA166301pde1_L6_Bpde1 L
SA166302pde2_H4_Bpde2 H
SA166303pde2_H6_Bpde2 H
SA166304pde2_H5_Bpde2 H
SA166305pde2_H3_Bpde2 H
SA166306pde2_H2_Bpde2 H
SA166307pde2_H1_Bpde2 H
SA166308pde2_L2_Bpde2 L
SA166309pde2_L1_Bpde2 L
SA166310pde2_L3_Bpde2 L
SA166311pde2_L4_Bpde2 L
SA166312pde2_L5_Bpde2 L
SA166313pde2_L6_Bpde2 L
SA166314ras1_H4_Bras1 H
SA166315ras1_H5_Bras1 H
SA166316ras1_H3_Bras1 H
SA166317ras1_H1_Bras1 H
SA166318ras1_H6_Bras1 H
SA166319ras1_H2_Bras1 H
SA166320ras1_L1_Bras1 L
SA166321ras1_L6_Bras1 L
SA166322ras1_L5_Bras1 L
SA166323ras1_L4_Bras1 L
SA166324ras1_L3_Bras1 L
SA166325ras1_L2_Bras1 L
SA166326ras2_H5_Bras2 H
SA166327ras2_H6_Bras2 H
SA166328ras2_H4_Bras2 H
SA166329ras2_H1_Bras2 H
SA166330ras2_H3_Bras2 H
SA166331ras2_H2_Bras2 H
SA166332ras2_L2_Bras2 L
SA166333ras2_L3_Bras2 L
SA166334ras2_L1_Bras2 L
SA166335ras2_L4_Bras2 L
SA166336ras2_L5_Bras2 L
SA166337ras2_L6_Bras2 L
SA166338rgs2_H5_Args2 H
SA166339rgs2_H6_Args2 H
SA166340rgs2_H3_Args2 H
SA166341rgs2_H4_Args2 H
SA166342rgs2_H2_Args2 H
SA166343rgs2_H1_Args2 H
SA166344rgs2_L4_Args2 L
SA166345rgs2_L5_Args2 L
SA166346rgs2_L3_Args2 L
SA166347rgs2_L1_Args2 L
SA166348rgs2_L6_Args2 L
SA166349rgs2_L2_Args2 L
SA166350rgt2_H1_Crgt2 H
SA166351rgt2_H6_Crgt2 H
SA166352rgt2_H4_Crgt2 H
SA166353rgt2_H5_Crgt2 H
SA166354rgt2_H3_Crgt2 H
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