Return to study ST001367 main page
MB Sample ID: SA099385
Local Sample ID: | MBGA_4 |
Subject ID: | SU001441 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | ~7 weeks (Pre-intervention) |
Gender: | Female |
Animal Animal Supplier: | Jackson Laboratories |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002275 | AN002276 | AN002277 | AN002278 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | HILIC | HILIC |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | Waters BEH C8 (50 x 2.1mm,1.7um) | Waters BEH C8 (50 x 2.1mm,1.7um) | Waters HILIC (100 x 2.1mm,1.8um) | Waters HILIC (100 x 2.1mm,1.8um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo LTQ-Orbitrap Velos Pro | Thermo LTQ-Orbitrap Velos Pro | Thermo LTQ-Orbitrap Velos Pro | Thermo LTQ-Orbitrap Velos Pro |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
Units | Abundance | abundance | Abundance | abundance |
MS:
MS ID: | MS002119 |
Analysis ID: | AN002275 |
Instrument Name: | Thermo LTQ-Orbitrap Velos Pro |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | For relative quantitation, the MS instrument was run in the survey scan mode with FTMS detection at a mass resolution of 60,000 FWHM at m/z 400. The mass scan range was m/z 80 to 1800, with a reference lock-mass for real-time calibration. Two UPLC-FTMS datasets were acquired for each sample, one with positive-ion detection and the other with negative-ion detection. LC-MS/MS data was also acquired from each sample set with collision induced dissociation (CID) at different levels of normalized collision energy (from publication methods). |
Ion Mode: | POSITIVE |
MS ID: | MS002120 |
Analysis ID: | AN002276 |
Instrument Name: | Thermo LTQ-Orbitrap Velos Pro |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | For relative quantitation, the MS instrument was run in the survey scan mode with FTMS detection at a mass resolution of 60,000 FWHM at m/z 400. The mass scan range was m/z 80 to 1800, with a reference lock-mass for real-time calibration. Two UPLC-FTMS datasets were acquired for each sample, one with positive-ion detection and the other with negative-ion detection. LC-MS/MS data was also acquired from each sample set with collision induced dissociation (CID) at different levels of normalized collision energy (from publication methods). |
Ion Mode: | NEGATIVE |
MS ID: | MS002121 |
Analysis ID: | AN002277 |
Instrument Name: | Thermo LTQ-Orbitrap Velos Pro |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The MS instrument was run in the survey scan mode with FTMS detection at a mass resolution of 60,000 FWHM at m/z 400. Two HILIC-FTMS datasets were acquired for each sample, one with positive-ion detection and the other with negative-ion detection. The mass scan range was m/z 80 to 800 (from publication methods). |
Ion Mode: | POSITIVE |
MS ID: | MS002122 |
Analysis ID: | AN002278 |
Instrument Name: | Thermo LTQ-Orbitrap Velos Pro |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The MS instrument was run in the survey scan mode with FTMS detection at a mass resolution of 60,000 FWHM at m/z 400. Two HILIC-FTMS datasets were acquired for each sample, one with positive-ion detection and the other with negative-ion detection. The mass scan range was m/z 80 to 800 (from publication methods). |
Ion Mode: | NEGATIVE |