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MB Sample ID: SA114974
| Local Sample ID: | PL3_HA_44243 |
| Subject ID: | SU001482 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002352 | AN002353 | AN002354 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity UPLC | Waters Acquity UPLC | Waters Acquity UPLC |
| Column | Waters Acquity UPLC BEH amide,(100 x 2.1mm,1.7um,100 Å) | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um,100 Å) | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um,100 Å) |
| MS Type | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | QTOF |
| MS instrument name | Waters Xevo G2-XS | Waters Xevo G2-XS | Waters Xevo G2-XS |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE |
| Units | normalized ion abundance | normalized ion abundance | normalized ion abundance |
MS:
| MS ID: | MS002194 |
| Analysis ID: | AN002352 |
| Instrument Name: | Waters Xevo G2-XS |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Mass spectrometry data was acquired using ‘sensitivity’ mode in positive and negative electrospray ionization mode within 50-1200 Da range. For the electrospray acquisition, the capillary voltage was set at 1.5 kV (positive), 3.0kV (negative), sample cone voltage 30V, source temperature at 120° C, cone gas flow 50 L/h and desolvation gas flow rate of 800 L/h with scan time of 0.5 sec in continuum mode. Leucine Enkephalin; 556.2771 Da (positive) and 554.2615 Da (negative) was used for lockspray correction and scans were performed at 0.5 min. The injection volume for each sample was 3µL, unless otherwise specified. The acquisition was carried out with instrument auto gain control to optimize instrument sensitivity over the samples acquisition time. LC-MS and LC-MSe data were processed using Progenesis QI (Nonlinear, Waters) and values were reported as area units. |
| Ion Mode: | POSITIVE |
| MS ID: | MS002195 |
| Analysis ID: | AN002353 |
| Instrument Name: | Waters Xevo G2-XS |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Mass spectrometry data was acquired using ‘sensitivity’ mode in positive and negative electrospray ionization mode within 50-1200 Da range. For the electrospray acquisition, the capillary voltage was set at 1.5 kV (positive), 3.0kV (negative), sample cone voltage 30V, source temperature at 120° C, cone gas flow 50 L/h and desolvation gas flow rate of 800 L/h with scan time of 0.5 sec in continuum mode. Leucine Enkephalin; 556.2771 Da (positive) and 554.2615 Da (negative) was used for lockspray correction and scans were performed at 0.5 min. The injection volume for each sample was 3µL, unless otherwise specified. The acquisition was carried out with instrument auto gain control to optimize instrument sensitivity over the samples acquisition time. LC-MS and LC-MSe data were processed using Progenesis QI (Nonlinear, Waters) and values were reported as area units. |
| Ion Mode: | POSITIVE |
| MS ID: | MS002196 |
| Analysis ID: | AN002354 |
| Instrument Name: | Waters Xevo G2-XS |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Mass spectrometry data was acquired using ‘sensitivity’ mode in positive and negative electrospray ionization mode within 100-2000 Da for complex lipids. For the electrospray acquisition, the capillary voltage was set at 1.5 kV (positive), 3.0kV (negative), sample cone voltage 30V, source temperature at 120° C, cone gas flow 50 L/h and desolvation gas flow rate of 800 L/h with scan time of 0.5 sec in continuum mode. Leucine Enkephalin; 556.2771 Da (positive) and 554.2615 Da (negative) was used for lockspray correction and scans were performed at 0.5 min. The injection volume for each sample was 3µL, unless otherwise specified. The acquisition was carried out with instrument auto gain control to optimize instrument sensitivity over the samples acquisition time. LC-MS and LC-MSe data were processed using Progenesis QI (Nonlinear, Waters) and values were reported as area units. |
| Ion Mode: | POSITIVE |
