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MB Sample ID: SA154564

Local Sample ID:KD-D3
Subject ID:SU001756
Subject Type:Cultured cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Not applicable

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Combined analysis:

Analysis ID AN002737
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity UHPLC UNSPSC 41115709
Column Waters BEH C18 (00mm x 2.1mm,1.7um )
MS Type ESI
MS instrument type QTOF
MS instrument name Bruker impact II UHPLC-QTOF system
Ion Mode POSITIVE
Units ng/ml

MS:

MS ID:MS002534
Analysis ID:AN002737
Instrument Name:Bruker impact II UHPLC-QTOF system
Instrument Type:QTOF
MS Type:ESI
MS Comments:The SM results for UGCG-silenced Th17 cells data was acquired on a UHPLC-QTOF system from Bruker (Bruker, Billerica, MA, USA) combining an Elute UHPLC binary pump and an Impact II system QTOF system. The samples for this experiments were the same extracts that the Cer data was acquired from and had SM(18:1/17:0) spiked in prior to acquisition. The data was acquired using the Hystar suite of software. MZmine 2 was used for all the untargeted data processing.
Ion Mode:POSITIVE
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