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MB Sample ID: SA174905
| Local Sample ID: | bdivrou_mero_3_met_1 |
| Subject ID: | SU001955 |
| Subject Type: | Other organism |
| Subject Species: | Babesia divergens |
| Taxonomy ID: | 32595 |
| Genotype Strain: | Rouen 1986 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003081 | AN003082 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
| Column | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6460 QQQ | Agilent 6460 QQQ |
| Ion Mode | NEGATIVE | NEGATIVE |
| Units |
MS:
| MS ID: | MS002827 |
| Analysis ID: | AN003081 |
| Instrument Name: | Agilent 6460 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Method 1: Data was acquired in MRM mode. To achieve a higher signal from low-abundance metabolites, transitions corresponding to metabolites of interests were separated in two MS methods (see method 2). Transitions utilized in this study are subtle modifications of the transition dataset contained in the Agilent dMRM database and method (see https://www.agilent.com/cs/library/technicaloverviews/public/5991-6482EN.pdf). Feature assignment was performed by matching transitions and RT with these contained in the Agilent dMRM Database and Method, and further confirmed by addition of authentic MS-grade standards. The software workflow included user visualization in Agilent MassHunter Workstation Software Qualitative Analysis (version B.09.00), and further compound integration using Agilent MassHunter Workstation Software Quantitative Analysis (version B.09.00). Only metabolites with signal-to-noise ratio higher than 3 (limit of detection) were reported. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS002828 |
| Analysis ID: | AN003082 |
| Instrument Name: | Agilent 6460 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Method 1: Data was acquired in MRM mode. To achieve a higher signal from low-abundance metabolites, transitions corresponding to metabolites of interests were separated in two MS methods (see method 2). Transitions utilized in this study are subtle modifications of the transition dataset contained in the Agilent dMRM database and method (see https://www.agilent.com/cs/library/technicaloverviews/public/5991-6482EN.pdf). Feature assignment was performed by matching transitions and RT with these contained in the Agilent dMRM Database and Method, and further confirmed by addition of authentic MS-grade standards. The software workflow included user visualization in Agilent MassHunter Workstation Software Qualitative Analysis (version B.09.00), and further compound integration using Agilent MassHunter Workstation Software Quantitative Analysis (version B.09.00). Only metabolites with signal-to-noise ratio higher than 3 (limit of detection) were reported. |
| Ion Mode: | NEGATIVE |
