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MB Sample ID: SA177866
| Local Sample ID: | 0060_LC_20190903_sample_0001 |
| Subject ID: | SU001997 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male and female |
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Combined analysis:
| Analysis ID | AN003118 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 6545 LC/QTOF |
| Column | Waters ACQUITY UPLC BEH C18 |
| MS Type | ESI |
| MS instrument type | GC-TOF |
| MS instrument name | Agilent 6545 LC/QTOF |
| Ion Mode | UNSPECIFIED |
| Units | Summarised value |
MS:
| MS ID: | MS002899 |
| Analysis ID: | AN003118 |
| Instrument Name: | Agilent 6545 LC/QTOF |
| Instrument Type: | GC-TOF |
| MS Type: | ESI |
| MS Comments: | The data processing included alignment of peaks, peak integration, normalization and identification. Lipids were identified using an internal spectral library. The data were normalized using one or more internal standards representative of each class of lipid present in the samples: the intensity of each identified lipid was normalized by dividing it with the intensity of its corresponding standard and multiplying it by the concentration of the standard. All monoacyl lipids except cholesterol esters, such as monoacylglycerols and monoacylglycerophospholipids, were normalized with PC(17:0/0:0), all diacyl lipids except ethanolamine phospholipids were normalized with PC(17:0/17:0), all ceramides with Cer(d18:1/17:0), all diacyl ethanolamine phospholipids with PE(17:0/17:0), and TG and cholesterol esters with TG(17:0/17:0/17:0). Other (unidentified) molecular species were normalized with PC(17:0/0:0) for retention times < 300 s, PC(17:0/17:0) for a retention time between 300 s and 410 s, and TG(17:0/17:0/17:0) for longer retention times. Quality control of the method showed that the day-to-day repeatability of control serum samples, and the relative standard deviation (RSD) for values identified was on average below 25% and 20% for discovery and validation sECs, respectively. The internal standards added to all samples in the study had an average RSD of 25% and 13 % in the discovery and validation sECs. |
| Ion Mode: | UNSPECIFIED |
