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MB Sample ID: SA183985
| Local Sample ID: | 5D_T4_I1_G2_L1 |
| Subject ID: | SU002028 |
| Subject Type: | Mammal |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Genotype Strain: | Sprague-Dawley |
| Age Or Age Range: | 8 weeks |
| Weight Or Weight Range: | 250-450 g |
| Gender: | Male and female |
| Animal Animal Supplier: | Charles River |
| Animal Light Cycle: | 12 h reverse light-dark cycles |
| Animal Feed: | ad libitum |
| Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003174 | AN003175 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Thermo Accucore C30 (50 x 2.1mm,2.1um) | Thermo Accucore C30 (50 x 2.1mm,2.1um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo ID-X Orbitrap Tribrid | Thermo ID-X Orbitrap Tribrid |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Normalized Peak Area | Normalized Peak Area |
MS:
| MS ID: | MS002952 |
| Analysis ID: | AN003174 |
| Instrument Name: | Thermo ID-X Orbitrap Tribrid |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | MS experiments were preformed over a scan range of 150-2000 m/z, maximum ion injection time was set to 200 ms, and orbitrap resolution was 24000. Raw spectral data from LC-MS experiments were pre-processed using Compound Discoverer v3.0.0 software (Thermo Fischer Scientific, Inc., Waltham, MA) and the XCMS web-based application (xcmsonline.scripps.edu). Initial steps involved retention time alignment between samples, peak area integration, peak picking, and QC area normalization. Features eluting with the solvent front or having retention times below 0.75 min were removed to account for potential ion suppression effects in that retention time region. ChemSpider and in-house mzVault database searches were used to obtain a list of tentative IDs based on accurate mass, isotope pattern, and MS/MS data whenever possible. Each lipid feature was identified according to the following confidence levels: (1) compounds matched to existing in house database standards by accurate mass (<2 ppm), isotopic abundance, fragmentation spectrum, and retention time; (2) compounds annotated according to accurate mass, isotopic abundance, and fragmentation consistent with Lipid Maps and Human Metabolome Database (HMDB) entries; (3) accurate mass match matched to Lipid Maps and HMDB entries and fragmentation showing a few matching characteristic fragment ions. |
| Ion Mode: | POSITIVE |
| Ion Source Temperature: | 275 ℃ |
| Ion Spray Voltage: | 3500 V |
| Source Temperature: | 320 ℃ |
| MS ID: | MS002953 |
| Analysis ID: | AN003175 |
| Instrument Name: | Thermo ID-X Orbitrap Tribrid |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | MS experiments were preformed over a scan range of 150-2000 m/z, maximum ion injection time was set to 200 ms, and orbitrap resolution was 24000. Raw spectral data from LC-MS experiments were pre-processed using Compound Discoverer v3.0.0 software (Thermo Fischer Scientific, Inc., Waltham, MA) and the XCMS web-based application (xcmsonline.scripps.edu). Initial steps involved retention time alignment between samples, peak area integration, peak picking, and QC area normalization. Features eluting with the solvent front or having retention times below 0.75 min were removed to account for potential ion suppression effects in that retention time region. ChemSpider and in-house mzVault database searches were used to obtain a list of tentative IDs based on accurate mass, isotope pattern, and MS/MS data whenever possible. Each lipid feature was identified according to the following confidence levels: (1) compounds matched to existing in house database standards by accurate mass (<2 ppm), isotopic abundance, fragmentation spectrum, and retention time; (2) compounds annotated according to accurate mass, isotopic abundance, and fragmentation consistent with Lipid Maps and Human Metabolome Database (HMDB) entries; (3) accurate mass match matched to Lipid Maps and HMDB entries and fragmentation showing a few matching characteristic fragment ions. |
| Ion Mode: | NEGATIVE |
| Ion Source Temperature: | 275 ℃ |
| Ion Spray Voltage: | -2500 V |
| Source Temperature: | 320 ℃ |
