Return to study ST001753 main page
MB Sample ID: SA185576
Local Sample ID: | Pooled Human Plasma 5 |
Subject ID: | SU002061 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Pooled |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002856 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Agilent 1290 Infinity II |
Column | Waters Acquity BEH Amide (100 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Agilent 6545 |
Ion Mode | POSITIVE |
Units | Total Intensity Units |
MS:
MS ID: | MS003005 |
Analysis ID: | AN002856 |
Instrument Name: | Agilent 6545 |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Instrument settings for positive ionization mode following electrospray ionization were as follows: Sheath gas flow rate, 11 L/min; Drying gas, 8 L/min; Drying gas temperature, 320 ºC; Nebulizer, 35 psi; Capillary voltage, 3500 V; Nozzle voltage, 1000 V; Fragmentor, 175 V; Skimmer, 65 V; Octupole 1 RF Vpp, 750 V; Collision energy, 20; Iterative MS/MS mass error tolerance, ± 20 ppm; Iterative MS/MS retention time exclusion tolerance, ± 0.5 min; Spectrum data type, centroid. Data de-pendent MS/MS parameters: Mass range, 25-1200 m/z; Rate, 2 spectra/s; Max precursor ions per cycle, 3; Absolute precursor threshold, 5000 counts; Relative precursor threshold, 0.001%; Active exclusion enabled after 2 spectra and released after 0.5 min; Isolation width, narrow (~1.3 m/z). Iterative LC-MS/MS data acquired from the modified HILIC separation conditions was loaded into a pre-release version of an in-house software tool, MetIDTracker for feature detection. This software uses MSPepSearch (NIST) for database searching and score assignment. |
Ion Mode: | POSITIVE |
Capillary Voltage: | 3500 V |
Collision Energy: | 20 |
Collision Gas: | N2 |
Dry Gas Flow: | 8L/min |
Dry Gas Temp: | 320 C |
Fragment Voltage: | 175 V |
Fragmentation Method: | CID |
Ionization: | ESI |
Mass Accuracy: | <20 ppm |