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MB Sample ID: SA186650
| Local Sample ID: | 3105170 |
| Subject ID: | SU002073 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male and female |
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Combined analysis:
| Analysis ID | AN003249 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters |
| Column | Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | NEGATIVE |
| Units | ng/ml |
MS:
| MS ID: | MS003022 |
| Analysis ID: | AN003249 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | The bile acids were measured in fecal sample as described based on . 20 μL of serum, using the same internal standard mixtures, was filtered through a frit filter plate (96-Well Protein Precipitation Filter Plate, Sigma Aldrich), and the effluent was collected and evaporated to dryness and the residue was dissolved in 20 μL of a 40:60 MeOH: H2O v/v mixture containing the same injection standards. Analyses were performed on an ACQUITY UPLC system coupled to a triple quadrupole mass spectrometer (Waters Corporation, Milford, USA) with an atmospheric electrospray interface operating in negative ion mode. An external calibration with six calibration points (0.5–600 ng/mL), including a solvent blank, was carried out for use in quantitation. |
| Ion Mode: | NEGATIVE |
