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MB Sample ID: SA194020

Local Sample ID:KR4M2
Subject ID:SU002143
Subject Type:Cultured cells
Subject Species:Mus musculus

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Combined analysis:

Analysis ID AN003360
Analysis type MS
Chromatography type HILIC
Chromatography system ThermoFisher Ultimate 3000 UHPLC system
Column Waters BEH Amide
MS Type ESI
MS instrument type Hybrid Quadrupole-Orbitrap™
MS instrument name ThermoFisher Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometry (QE)
Ion Mode NEGATIVE
Units pmoles/I

MS:

MS ID:MS003129
Analysis ID:AN003360
Instrument Name:ThermoFisher Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometry (QE)
Instrument Type:Hybrid Quadrupole-Orbitrap™
MS Type:ESI
MS Comments:The eluents were analyzed on a ThermoFisher Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometry (QE) in Heated Electrospray Ionization Negative (HESI-) mode, separately. Spray voltage was set to 4000 V. Capillary and Probe Heater Temperature were separately 320 °C and 320 °C. Sheath gas flow rate was 35 (Arb, arbitrary unit), and Aux gas flow rate was 10 (Arb). S-Lens RF Level was 50 (Arb). The full scan was operated at a high-resolution of 70000 FWHM (m/z=200) at a range of 70- 1050 m/z with AGC Target setting at 3×106.
Ion Mode:NEGATIVE
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