Return to study ST002091 main page
MB Sample ID: SA200762
| Local Sample ID: | 92 |
| Subject ID: | SU002175 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 36-78 |
| Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003415 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | Sciex 5500 |
| Column | none |
| MS Type | ESI |
| MS instrument type | QTRAP |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE |
| Units | nmol/ml |
MS:
| MS ID: | MS003180 |
| Analysis ID: | AN003415 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Hardware/System Setup: The Lipidyzer platform hardware has been described previously. (10) The Turbo V source is outfitted with a 65 μm ESI Electrode and the transfer line is plumbed with 50 μm ID PEEKsil tubing from the Lipidyzer installation kit. For the SLA application method, a 100 μL loop was installed. SLA Overview: The Shotgun Lipidomics Assistant (SLA) oversees three processes in the DMS lipidomics workflow: (1) analyzing DMS tuning data to update methods utilizing the DMS, (2) analyzing QC/suitability test data to measure relative lipid standard response, and (3) analyzing DMS lipidomics data files to yield processed quantitative results. This third process is subdivided into three parts: (1) extracting data from Analyst data files, (2) merging extracted data with the sample map and performing normalization, and (3) creating plots and data sheets of the class total and triacylglycerol data. These five processes are carried out on separate tabs within the SLA GUI. We will describe each of these five processes and the computations performed in detail below. While the LWM scripted the creation of Analyst sample batches and performed data analysis for these three processes, our workflow allows the user to setup the batches manually in Analyst and performs the analysis on the resulting data. This manual batch creation step requires a minimal amount of user time and gives the user flexibility in performing tuning, QC/suitability tests, and analyzing experimental data. The first step of analysis within each of the three primary processes is the conversion of the WIFF files to mzML format using Proteowizard’s MSconvertGUI application. (11) The SLA reads the resulting mzML files utilizing the pyOpenMS package (12) and converts the data into readable data tables for further data processing. Please refer to the following publication for more details: https://pubmed.ncbi.nlm.nih.gov/34637296/ |
| Ion Mode: | POSITIVE |
