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MB Sample ID: SA205312
| Local Sample ID: | T15_11 |
| Subject ID: | SU002222 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003498 |
|---|---|
| Analysis type | MS |
| Chromatography type | CE |
| Chromatography system | ZipChip (908 Devices) |
| Column | HS chip |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Exactive Plus Orbitrap |
| Ion Mode | POSITIVE |
| Units | Micromolars |
MS:
| MS ID: | MS003258 |
| Analysis ID: | AN003498 |
| Instrument Name: | Thermo Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | µCE-HRMS analyses were performed using the microchip ZipChip® capillary electrophoresis system (classic interface, 908 Devices, Boston MA), coupled to a high-resolution accurate mass Q Exactive plus mass spectrometer (Thermo Fisher Scientific, MA). µCE-HRMS separations used ZipChip® HS chips (10 cm channel). All experiments were performed in positive ionization mode in the 50-500 m/z range at a mass resolution setting of 17,500. The capillary temperature was set to 200 °C and the sheath gas flow rate was 2 psi. The automatic gain control (AGC) target value was set to 3E6 and the maximum injection time was 20 ms. Data were acquired using Xcalibur 3.0 (Thermo Scientific) and were imported to Skyline software14 for peak picking and integration. The peak picking procedure used the analyte accurate m/z and migration time. Peak areas obtained from Skyline were exported as spreadsheets for further analysis. Quantitation was performed with the analyte peak areas relative to the peak area of one of the three isotopically labeled internal standards (13C6 arginine, 13C methionine D3 and 13C phenylalanine) chosen based on migration time similarities |
| Ion Mode: | POSITIVE |
