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MB Sample ID: SA206920
| Local Sample ID: | 190905_19_0009_UCLM_CCM_009 |
| Subject ID: | SU002243 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Male |
| Cell Biosource Or Supplier: | CLS |
| Cell Strain Details: | In vitro spontaneously transformed keratinocytes from histologically normal skin |
| Cell Primary Immortalized: | HaCaT |
| Cell Passage Number: | Up to the 15th passage |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003532 |
|---|---|
| Analysis type | MS |
| Chromatography type | Ion pair |
| Chromatography system | Waters Acquity H-Class |
| Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Time of flight |
| MS instrument name | Waters LCT premier |
| Ion Mode | NEGATIVE |
| Units | Log2 (fold-change) |
MS:
| MS ID: | MS003290 |
| Analysis ID: | AN003532 |
| Instrument Name: | Waters LCT premier |
| Instrument Type: | Time of flight |
| MS Type: | ESI |
| MS Comments: | The eluent was introduced into the mass spectrometer (LCT Premier (Waters Corp.) by electrospray ionization, with capillary and cone voltages set in the negative ion mode to 2800 and 100 V, respectively. The nebulization gas was set to 600 L/hour and 300ºC. The cone gas was fixed at 50 L/hour, and the source temperature was maintained at 120ºC. Centroid data were acquired over the mass range of 50-1000 Da, using an accumulation time of 0.2 seconds per spectrum. All spectra were mass corrected in real time by reference to leucine enkephalin, infused at 10 µL/minute through an independent reference electrospray, sampled every 10 seconds. |
| Ion Mode: | NEGATIVE |
