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MB Sample ID: SA207440
| Local Sample ID: | HFD-HV_45_12122 |
| Subject ID: | SU002247 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003540 | AN003541 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | IR-MALDI |
| Chromatography system | Thermo Vanquish | Other |
| Column | Waters XBridge BEH Amide (150mm x 2.1mm,2.5um) | Other |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Exploris 240 Orbitrap |
| Ion Mode | NEGATIVE | NEGATIVE |
| Units | Total Abundance | Peak Area |
MS:
| MS ID: | MS003298 |
| Analysis ID: | AN003540 |
| Instrument Name: | Thermo Q Exactive Plus Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | Fullscan mass spectrometry. The full scan mass spectrometry analysis was performed on a Thermo Q Exactive PLUS with a HESI source which was set to a spray voltage of -2.7kV under negative mode and 3.5kV under positive mode. The sheath, auxiliary, and sweep gas flow rates of 40, 10, and 2 (arbitrary unit), respectively. The capillary temperature was set to 300°C, and the aux gas heater was 360°C. The S-lens RF level was 45. The m/z range was set to 72 to 1000 m/z under both positive and negative ionization mode. The AGC target was set to 3e6, and the maximum IT was 200 ms. The mass resolution (full-width half maximum) was set to 70,000 @ m/z = 200. |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | cwwong_methods.pdf |
| MS ID: | MS003299 |
| Analysis ID: | AN003541 |
| Instrument Name: | Thermo Exploris 240 Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | To ablate targeted tissue regions a 2970-nm wavelength laser was used with a single burst of ten pulses to produce 1 mJ of energy at a rate of 10 kHz. X and Y stage movements of 100 µm were used to achieve oversampling(Nazari and Muddiman, 2015). Ablated analytes were post-ionized in the orthogonal electrospray plume, established by applying a voltage of approximately 3 kV to the electrospray solvent (1 mM acetic acid in 50:50 water/acetonitrile.) Mass spectrometry analysis of ionized molecules was performed in negative mode with internal calibrant used to achieve high mass accuracy (<2.5 ppm) within the 85-225 m/z range. Automatic gain control (AGC) was disabled. A mass resolution power of 240,000FWHM at 200 m/z was used with a fixed injection time (15 ms) to synchronize timing of the ablation plume with ion collection in the C-trap of the mass spectrometer. |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | cwwong_methods.pdf |
