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MB Sample ID: SA209347

Local Sample ID:154
Subject ID:SU002266
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male and female

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN003570 AN003571
Analysis type MS MS
Chromatography type Flow induction analysis Flow induction analysis
Chromatography system Shimazdu LC-30AD Shimazdu LC-30AD
Column none none
MS Type ESI ESI
MS instrument type QTRAP QTRAP
MS instrument name ABI Sciex 5500 QTrap ABI Sciex 5500 QTrap
Ion Mode UNSPECIFIED UNSPECIFIED
Units nmol/g nmol/g

MS:

MS ID:MS003327
Analysis ID:AN003570
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:QTRAP
MS Type:ESI
MS Comments:The Lipidyzer (SCIEX), a QTRAP system with SelexION ion mobility, was used for targeted profiling as described previously (Contrepois et al., 2018). In brief, flow injection analysis was performed with a LC-30AD (Shimazdu) operating at 8 μL/min (50 μL injection volume) using a running solution that consisted of 10 mM ammonium acetate in dichloromethane:MeOH (50:50). DMS separates lipids based on the principle that each lipid class has a different head group dipole moment and thus mobility in the DMS aperture (Schneider et al., 2010). The lipid molecular species were identified and quantified using multiple reaction monitoring (MRM) and positive/negative switching. Two acquisition methods were employed covering 10 lipid classes across positive and negative mode. Method 1 had SelexION voltages turned on while Method 2 had SelexION voltages turned off. Method 1 employed an isocratic flow of 8 μL/min for 7.9 min, followed by a 2 minute wash at 30 μL/min. Method 2 employed an isocratic flow of 8 μL/min for 6 minutes, followed by 2 minute wash at 30 μL/min. Each lipid was acquired throughout 20 cycles. Lipid classes targeted in positive mode: SM, DAG, CE, CER, and TAG. Lipid classes targeted in negative mode: LPE, LPC, PC, PE, and FFA. Lipids were quantified using the LWM software, which compares endogenous lipids to the known concentrations of structurally most similar spiked-in lipid standards and reports all detected lipids in nmol/g. Data analysis. Data were downloaded from the Lipidyzer LWM and merged and processed in R. In brief, Excel files (LWM output) were read with the “loadWorkbook” package. From all samples, lipid concentrations determined in a blank control (sample processed in parallel without the addition of cells) were subtracted to correct for background signals. The data set was further filtered accepting only lipid species that detected in at least 25% of all samples. Missing values were imputed by drawing from a random distribution.
Ion Mode:UNSPECIFIED
  
MS ID:MS003328
Analysis ID:AN003571
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:QTRAP
MS Type:ESI
MS Comments:The Lipidyzer (SCIEX), a QTRAP system with SelexION ion mobility, was used for targeted profiling as described previously (Contrepois et al., 2018). In brief, flow injection analysis was performed with a LC-30AD (Shimazdu) operating at 8 μL/min (50 μL injection volume) using a running solution that consisted of 10 mM ammonium acetate in dichloromethane:MeOH (50:50). DMS separates lipids based on the principle that each lipid class has a different head group dipole moment and thus mobility in the DMS aperture (Schneider et al., 2010). The lipid molecular species were identified and quantified using multiple reaction monitoring (MRM) and positive/negative switching. Two acquisition methods were employed covering 10 lipid classes across positive and negative mode. Method 1 had SelexION voltages turned on while Method 2 had SelexION voltages turned off. Method 1 employed an isocratic flow of 8 μL/min for 7.9 min, followed by a 2 minute wash at 30 μL/min. Method 2 employed an isocratic flow of 8 μL/min for 6 minutes, followed by 2 minute wash at 30 μL/min. Each lipid was acquired throughout 20 cycles. Lipid classes targeted in positive mode: SM, DAG, CE, CER, and TAG. Lipid classes targeted in negative mode: LPE, LPC, PC, PE, and FFA. Lipids were quantified using the LWM software, which compares endogenous lipids to the known concentrations of structurally most similar spiked-in lipid standards and reports all detected lipids in nmol/g. Data analysis. Data were downloaded from the Lipidyzer LWM and merged and processed in R. In brief, Excel files (LWM output) were read with the “loadWorkbook” package. From all samples, lipid concentrations determined in a blank control (sample processed in parallel without the addition of cells) were subtracted to correct for background signals. The data set was further filtered accepting only lipid species that detected in at least 25% of all samples. Missing values were imputed by drawing from a random distribution.
Ion Mode:UNSPECIFIED
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