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MB Sample ID: SA212660

Local Sample ID:WT_EV1_SR
Subject ID:SU002318
Subject Type:Yeast
Subject Species:Saccharomyces cerevisiae
Taxonomy ID:4932
Genotype Strain:BY4743 (MATa/\textalpha, his3/his3, leu2/leu2, ura3/ura3, met15/MET15, lys2/LYS2)

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Combined analysis:

Analysis ID AN003642
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Dionex Ultimate 3000
Column Waters XBridge Amide (100 x 4.6mm,3.5um)
MS Type ESI
MS instrument type LC-Q Exactive
MS instrument name Q Exactive
Ion Mode UNSPECIFIED
Units peak height

MS:

MS ID:MS003393
Analysis ID:AN003642
Instrument Name:Q Exactive
Instrument Type:LC-Q Exactive
MS Type:ESI
MS Comments:Data were collected, metabolites were identified, and their peak area was recorded using El-MAVEN software [Agrawal et al., Methods Mol. Biol., 2019; Clasquin et al., Curr. Protoc. Bioinformatics, 2012; Melamud et al., Anal. Chem., 2010]. These data were transferred to an Excel spreadsheet (Microsoft, Redmond WA). Metabolite identity was established using a combination of an in-house metabolite library developed using pure purchased standards, the NIST (https://www.nist.gov) and Fiehn libraries [Kind et al, Anal. Chem., 2009]. P-values were derived using a homoscedastic, two-tailed Student's T-test and adjusted using the Benjamini-Hochberg correction procedure.
Ion Mode:UNSPECIFIED
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