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MB Sample ID: SA216912
| Local Sample ID: | QC08 |
| Subject ID: | SU002338 |
| Subject Type: | Plant |
| Subject Species: | Arabidopsis thaliana |
| Taxonomy ID: | 3702 |
| Genotype Strain: | WT Col-0, atg7-2 (GABI_655B06), atg9-4 (SALK_145980) |
| Age Or Age Range: | 11-day old |
| Gender: | Not applicable |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003679 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | N/A |
| Column | N/A |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Waters Xevo-TQ-S |
| Ion Mode | POSITIVE |
| Units | nmol/mg dry weight |
MS:
| MS ID: | MS003430 |
| Analysis ID: | AN003679 |
| Instrument Name: | Waters Xevo-TQ-S |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Mass spectrometry on a Xevo TQ-S (Waters Co., Milford, MA) was used to analyze the intact lipids by direct infusion in positive ion mode with precursor and neutral loss scans (Peters, Li et al. 2010, Xiao, Gao et al. 2010, Li, Baughman et al. 2014), using the scans shown in the Table. Data processing was also performed as previously described. Response factors were applied to the MGDG and DGDG analyses to correct for differences in the response of the mass spectrometer to unsaturated galactolipid species compared to the saturated internal standards. Generally, phospholipid data do not require response factor corrections, as the biological compounds and the internal standard have similar response factors (and no corrections were applied). |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | Lipidomics_MS.pdf |
