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MB Sample ID: SA232163

Local Sample ID:S20
Subject ID:SU002423
Subject Type:Invertebrate
Subject Species:Drosophila melanogaster
Taxonomy ID:7227
Age Or Age Range:15 day old flies
Gender:Male and female

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Combined analysis:

Analysis ID AN003814
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system none
Column none
MS Type ESI
MS instrument type QTRAP
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode POSITIVE
Units pMol

MS:

MS ID:MS003556
Analysis ID:AN003814
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:QTRAP
MS Type:ESI
MS Comments:Samples are analyzed in both positive and negative ion modes, with MS resolution Rm/z=200=280000 and MSMS resolution Rm/z=200=17500, in a single acquisition. Acquired data was processed using lipid identification software based on LipidXplorer (1). Data post-processing and normalization were performed by Lipotype using a developed data management system. Data analysis was performed using GraphPad Prism software 9.3.1. Pmol values (obtained by MS) of individual lipid species were transformed into a fraction of the total PA, DAG, PI, PC, PE, and PS lipids in the sample. (1)Herzog, R. et al. Lipidxplorer: A software for consensual cross-platform lipidomics. PLoS ONE 7, e29851 (2012).
Ion Mode:POSITIVE
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