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MB Sample ID: SA237295
| Local Sample ID: | m328 |
| Subject ID: | SU002458 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | wild type C57BL6/J |
| Age Or Age Range: | 8 weeks |
| Weight Or Weight Range: | 25-30g |
| Gender: | Male |
| Animal Animal Supplier: | Animal Resource Centre, Western Australai |
| Animal Housing: | standard |
| Animal Light Cycle: | 12 h light dark cycle |
| Animal Feed: | normal chow |
| Animal Water: | autoclaved |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003864 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | Shimadzu TQ8050NX |
| Column | Agilent DB5-MS (30m) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Shimadzu TQ8050NX |
| Ion Mode | UNSPECIFIED |
| Units | area |
MS:
| MS ID: | MS003605 |
| Analysis ID: | AN003864 |
| Instrument Name: | Shimadzu TQ8050NX |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | The GC-MS system used comprised of an AOC6000 autosampler, a 2030 Shimadzu gas chromatograph and a TQ8050NX triple quadrupole mass spectrometer (Shimadzu, Japan). The mass spectrometer was tuned according to the manufacturer’s recommendations using tris-(perfluorobutyl)-amine (CF43). GC-MS was performed on a 30m Agilent DB-5 column with 0.25mm internal diameter column and 1µm film thickness. The injection temperature (inlet) was set at 280°C, the MS transfer line at 280°C and the ion source adjusted to 200°C. Helium was used as the carrier gas at a flow rate of 1 mL/min and argon gas was used in the collision cell to generate the MRM product ion. The analysis of TMS samples was performed under the following oven temperature program; 100°C start temperature, hold for 4 minutes, followed by a 10°C min-1 oven temperature ramp to 320°C with a following final hold for 11 minutes. Approximately 520 targets were collected using the Shimadzu Smart Metabolite Database, where each target comprised a quantifier MRM along with a qualifier MRM, which covers approximately 350 endogenous metabolites and multiple stable isotopically labelled internal standards. Resultant data was processed using Shimadzu LabSolutions Insight software, where peak integrations were visually validated and manually corrected where required. |
| Ion Mode: | UNSPECIFIED |
