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MB Sample ID: SA239104

Local Sample ID:CTL_03_LIP329
Subject ID:SU002489
Subject Type:Cultured cells
Subject Species:Homo sapiens

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Combined analysis:

Analysis ID AN003907 AN003908 AN003909 AN003910 AN003911
Analysis type MS MS MS MS MS
Chromatography type HILIC HILIC HILIC Reversed phase Reversed phase
Chromatography system Shimadzu 20AD Shimadzu 20AD Shimadzu 20AD Shimadzu 20AD Shimadzu 20AD
Column Phenomenex Kinetex HILIC (150 x 3mm,2.6um) Phenomenex Kinetex HILIC (150 x 3mm,2.6um) Phenomenex Kinetex HILIC (150 x 3mm,2.6um) Merck Supelco Ascentis Express C18 (150 x 2.1mm,2.7um) Merck Supelco Ascentis Express C18 (150 x 2.1mm,2.7um)
MS Type ESI ESI ESI ESI ESI
MS instrument type QTRAP QTRAP QTRAP QTRAP QTRAP
MS instrument name ABI Sciex 4000 QTrap ABI Sciex 4000 QTrap ABI Sciex 4000 QTrap ABI Sciex 4000 QTrap ABI Sciex 4000 QTrap
Ion Mode POSITIVE POSITIVE POSITIVE POSITIVE POSITIVE
Units mol% of total lipids mol% of total lipids mol% of total lipids mol% of total lipids mol% of total lipids

MS:

MS ID:MS003646
Analysis ID:AN003907
Instrument Name:ABI Sciex 4000 QTrap
Instrument Type:QTRAP
MS Type:ESI
MS Comments:MRM acquisition of broad chromatographic peaks of low abundant phospho- and sphingolipid classes: PS, PA, LPC, LPE and some ceramides this acquisition is called "long_1x"
Ion Mode:POSITIVE
  
MS ID:MS003647
Analysis ID:AN003908
Instrument Name:ABI Sciex 4000 QTrap
Instrument Type:QTRAP
MS Type:ESI
MS Comments:MRM acquisition of narrow chromatographic peaks of low abundant phospho- and sphingolipid classes: cer, PG, PI, PE, PE-P This acquisition is referred to as "short_1x"
Ion Mode:POSITIVE
  
MS ID:MS003648
Analysis ID:AN003909
Instrument Name:ABI Sciex 4000 QTrap
Instrument Type:QTRAP
MS Type:ESI
MS Comments:MRM acquisition of narrow chromatographic peaks of abundant phospho- and sphingolipid classes: PC and SM following 20-fold dilution This acquisition is referred to as "short_20x"
Ion Mode:POSITIVE
  
MS ID:MS003649
Analysis ID:AN003910
Instrument Name:ABI Sciex 4000 QTrap
Instrument Type:QTRAP
MS Type:ESI
MS Comments:MRM acquisition of low abundant neutral lipids: DG This acquisition is referred to as "C18_1x"
Ion Mode:POSITIVE
  
MS ID:MS003650
Analysis ID:AN003911
Instrument Name:ABI Sciex 4000 QTrap
Instrument Type:QTRAP
MS Type:ESI
MS Comments:MRM acquisition of abundant neutral lipids: CE and TG after 10 fold dilution This acquisition is referred to as "C18_10x"
Ion Mode:POSITIVE
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