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MB Sample ID: SA241946
| Local Sample ID: | F2-27-03 |
| Subject ID: | SU002502 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003933 | AN003934 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area | peak area |
MS:
| MS ID: | MS003671 |
| Analysis ID: | AN003933 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | the mass spectrometer operated in top 10 data dependent MS2 mode with NCE of 30 eV (HCD) and dynamic exclusion of 10 s. MS1 scans were acquired at a resolution of 70,000 across the m/z range of 125-1500. MS2 scans were acquired at a resolution of 17,500. Sheath and auxiliary gases (both N2) were set at 45 and 15 (unitless), respectively. Instrument stability and quality control were assessed via injection of technical replicates every 6 runs. Untargeted lipid results were obtained using LipidSearch (Thermo) with precursor ion tolerance set to 5 ppm and product ion tolerance at 8 ppm. |
| Ion Mode: | NEGATIVE |
| MS ID: | MS003672 |
| Analysis ID: | AN003934 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | the mass spectrometer operated in top 10 data dependent MS2 mode with NCE of 30 eV (HCD) and dynamic exclusion of 10 s. MS1 scans were acquired at a resolution of 70,000 across the m/z range of 125-1500. MS2 scans were acquired at a resolution of 17,500. Sheath and auxiliary gases (both N2) were set at 45 and 15 (unitless), respectively. Instrument stability and quality control were assessed via injection of technical replicates every 6 runs. Untargeted lipid results were obtained using LipidSearch (Thermo) with precursor ion tolerance set to 5 ppm and product ion tolerance at 8 ppm. |
| Ion Mode: | POSITIVE |
