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MB Sample ID: SA245714
| Local Sample ID: | CO_FFA_7 |
| Subject ID: | SU002544 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 6 weeks |
| Gender: | Female |
| Animal Animal Supplier: | JAX |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004005 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu Nexera X2 |
| Column | Phenomenex Kinetex C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 4000 QTrap |
| Ion Mode | UNSPECIFIED |
| Units | intensity |
MS:
| MS ID: | MS003753 |
| Analysis ID: | AN004005 |
| Instrument Name: | ABI Sciex 4000 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | The samples were applied to a Kinetex C18 column (Kinetex C18, 2.1 x 150 mm, 1.7 µm particle; Phenomenex, Inc., Torrance, CA, USA) connected with ESI-MS/MS on a liquid chromatography (NexeraX2 system; Shimadzu Co., Kyoto, Japan) coupled with a 4000Q-TRAP quadrupole-linear ion trap hybrid mass spectrometer (AB Sciex, Framingham, MA, USA). For analyses of free fatty acids (FFAs), lysophospholipids (LPLs) and phospholipids, the samples were applied to the column and separated by a step gradient with mobile phase A (acetonitrile/methanol/water =1/1/1 (v/v/v) containing 5 mM phosphoric acid and 1 mM ammonium formate) and mobile phase B (2-propanol containing 5 µM phosphoric acid and 1 mM ammonium formate) at a flow rate of 0.2 mL/min at 50°C. For analyses of oxygenated fatty acid metabolites, the samples were applied to the column and separated using a step gradient including mobile phase C (water containing 0.1 % acetic acid) and mobile phase D (acetonitrile/methanol = 4/1 (v/v)) at a flow rate of 0.2 mL/ min at 45°C. Identification of phospholipids, LPLs, FFAs, and oxygenated PUFAs (polyunsaturated fatty acids) metabolites was conducted by multiple reaction monitoring (MRM) transition, and quantification was performed based on the peak area of the MRM transition and the calibration curve obtained with an authentic standard for each compound. |
| Ion Mode: | UNSPECIFIED |
