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MB Sample ID: SA247299
| Local Sample ID: | PCC11801 60 SEC SWATH1-1 BR1 |
| Subject ID: | SU002556 |
| Subject Type: | Bacteria |
| Subject Species: | Synechococcus elongatus |
| Taxonomy ID: | 2219813 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004022 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Shimadzu 20AD |
| Column | Phenomenex Synergi Hydro RP 100 A (100 x 2mm, 2.5um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF |
| Ion Mode | NEGATIVE |
| Units | Relative Abundance of Isotopologues |
MS:
| MS ID: | MS003769 |
| Analysis ID: | AN004022 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The data was acquired with SWATH methods. The details of SWATH methods can be found in the article https://doi.org/10.1016/j.isci.2020.101704. The quantification of MID of metabolites and fragment ions was performed by integrating the area under the curve of precursor and fragment isotopologues using MultiQuant 3.0.1 (Sciex, Framingham, MA) from the Q1 isolation window of the SWATH program, where all the precursor isotopologues of the respective metabolite were present. The peak areas were used to estimate precursor and product MID: mi=Mi/(∑(j=0)^n Mj ) where mi and Mi represent the normalized relative and unnormalized isotopologue abundance for each precursor/fragment ion in which i 13C atoms are incorporated, and n represents the number of carbon. |
| Ion Mode: | NEGATIVE |
