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MB Sample ID: SA247792
| Local Sample ID: | 36-week db/db+aav9-RDH10-3 |
| Subject ID: | SU002564 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | db/m; db/db; db/db+retinol; db/db+retinoic acid; db/db+AAV9-RDH10; RDH10-cKO; RDH10fl/fl |
| Age Or Age Range: | 32-week; 36-week; 21-week |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004041 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Sciex Triple Quad TM 4500MD MS |
| Column | Phenomenex Kinetex C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTRAP |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE |
| Units | ng/ml |
MS:
| MS ID: | MS003788 |
| Analysis ID: | AN004041 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | The MS conditions were as follows: electrospray ionization (ESI) under positive mode; nebulizer gas: nitrogen; curtain gas,30 psi; ion spray voltage, 4000V; temperature, 400ºC; gas 1 and gas 2, 35 and 40 psi, respectively; collision gas, 10 psi. The parameters of the mass spectrometer were optimized, and the multiple reaction monitoring (MRM) transitions of retinol, all-trans-retinoic acid, as well as D4-retinol were chosen as 269.2>93.1, 273.1>94.0, and 301.2>123.1, respectively. The MRM transitions for retinyl esters were chosen as 329.3>269.3 for retinyl acetate, 524.4>268.1 for retinyl palmitate (16:0), 552.5>268.2 for retinyl stearate (18:0), 522.4>268, retinyl palmitoleate (16:1), and retinyl oleate (18:1). The quantification was performed using the calibration curve. |
| Ion Mode: | POSITIVE |
