Return to study ST002515 main page
MB Sample ID: SA253162
| Local Sample ID: | P1-G08 |
| Subject ID: | SU002615 |
| Subject Type: | Bacteria |
| Subject Species: | Eggerthella lenta |
| Taxonomy ID: | 84112 |
| Genotype Strain: | DSM 2243, Valencia, AB8n2 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004142 |
|---|---|
| Analysis type | MS |
| Chromatography type | Unspecified |
| Chromatography system | Agilent 1290 Infinity II |
| Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6545 QTOF |
| Ion Mode | NEGATIVE |
| Units | mM |
MS:
| MS ID: | MS003889 |
| Analysis ID: | AN004142 |
| Instrument Name: | Agilent 6545 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Samples were injected via refrigerated autosampler into mobile phase and chromatographically separated by an Agilent 1290 Infinity II UPLC and detected using an Agilent 6545XT Q-TOF (quadrupole time of flight) mass spectrometer equipped with a dual jet stream electrospray ionization source, operating under extended dynamic range (1,700 m/z). MS1 spectra were collected in centroid mode, and peak assignments in samples were made based on comparisons of retention times and accurate masses from authentic standards using MassHunter Quantitative Analysis v.10.0 software from Agilent Technologies. Acetate was quantified from calibration curves constructed with acetate-d4 as a standard using isotope-dilution MS with phenylpropionate-d9 as the internal standard. Calibration curves were performed in a modified base form of EDM1 lacking amino acids and other carboxylic acids. A background level of 1.05mM of acetate was subtracted to obtain the final quantities. |
| Ion Mode: | NEGATIVE |
