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MB Sample ID: SA254543
| Local Sample ID: | CTX T1DM 1 |
| Subject ID: | SU002625 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004159 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975C |
| Ion Mode | POSITIVE |
| Units | Relative abundance |
MS:
| MS ID: | MS003906 |
| Analysis ID: | AN004159 |
| Instrument Name: | Agilent 5975C |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | GCMS protocols were similar to those described previously with a modified temperature gradient was used for GC: Initial temperature was 130degC, held for 4 minutes, rising at 6degC/minutes to 243degC, rising at 60degC/minutes to 280degC, held for 2 minutes. The electron ionization (EI) energy was set to 70 eV. Scan (m/z:50-800) and full scan mode were used for metabolomics analysis. Mass spectra were translated to relative metabolite abundance using the Automated Mass Spectral Deconvolution and Identification System (AMDIS) software matched to the FiehnLib metabolomics library (available through Agilent) for retention time and fragmentation pattern matching with a confidence score of > 80 (Fiehn, 2016; Fiehn et al., 2000; Kind et al., 2009). Data was further analyzed using the Data Extraction for Stable Isotope-labelled Metabolites (DEXSI) software package. Untargeted metabolomics data was normalized to total ion chromatogram. For glucose tracer raw data was exported and correction for natural abundance was done by IsoCorrectoR. Fractional enrichment of each metabolite was calculated as the relative abundance of each isotopologue relative to the sum of all other isotopologues. Mean enrichment was calculated as sum of fractional enrichment of labeled isotopologues (M1, M2, M3…). For principal component analysis, pathway impact analysis the online tool Metaboanlyst was used (https://www.metaboanalyst.ca/). Data was auto scaled and log transformed. |
| Ion Mode: | POSITIVE |
