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MB Sample ID: SA254928
| Local Sample ID: | 1-1_nontumor |
| Subject ID: | SU002634 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004169 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | SeQuant ZIC-HILIC (150 x 2.1mm,5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Orbitrap ID-X tribrid |
| Ion Mode | UNSPECIFIED |
| Units | Peak areas |
MS:
| MS ID: | MS003916 |
| Analysis ID: | AN004169 |
| Instrument Name: | Thermo Orbitrap ID-X tribrid |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | A Vanquish UHPLC system was coupled to an Orbitrap ID-X Tribrid mass spectrometer (Thermo Fisher Scientific) via electrospray ionization with the following source conditions: sheath gas flow 50 arbitrary units (Arb), auxiliary gas flow 10 Arb, sweep gas flow 1 Arb, ion transfer tube temperature 300 °C, vaporizer temperature 200 °C respectively. The RF lens value was 60%. Data were acquired in negative and positive polarity with a spray voltage of 2.8 kV and 3.5 kV, respectively. MS1 data were acquired from 67-900 m/z at a resolution of 120,000 with an automatic gain control (AGC) target of 2e5 and a maximum injection time of 200 ms in polarity switching mode. MS2 data for metabolite identification were acquired at a resolution of 15,000 with an AGC target of 2.5e4 and a maximum injection time of 70 ms in negative and positive mode separately. A 5 ppm mass error and 10 s dynamic exclusion were applied. |
| Ion Mode: | UNSPECIFIED |
