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MB Sample ID: SA256823
| Local Sample ID: | sample_0084 |
| Subject ID: | SU002655 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Not applicable |
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Combined analysis:
| Analysis ID | AN004206 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity II |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6545 QTOF |
| Ion Mode | NEGATIVE |
| Units | ng/ml |
MS:
| MS ID: | MS003953 |
| Analysis ID: | AN004206 |
| Instrument Name: | Agilent 6545 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The analyses were done using UHPLC-QTOFMS instrument from Agilent Technologies (Santa Clara, CA, USA). The analysis was done on an ACQUITY UPLC® BEH C18 column (2.1 mm × 100 mm, particle size 1.7 µm) by Waters (Milford, MA, USA). Mobile phase A consisted of H2O:MeOH (v/v 70:30) and mobile phase B of MeOH with both phases containing 2mM ammonium acetate as an ionization agent. The LC pump was programmed at a flow rate of 0.4 mL/min with the elution gradient as follows: from min 0-1.5, the percentage of phase B was increased from 5% to 30%, from min 1.5-4.5, the percentage of B was increased to 70%, from min 4.5-7.5 the percentage of B was increased to 100% and held for 5.5 mins. A post-time of 5 min was used to regain the initial conditions for the next analysis. The total analysis time per sample was 18 min. The dual ESI ionization source was settings were as follows: capillary voltage was 4.5 kV, nozzle voltage 1500 V, N2 pressure in the nebulized was 21 psi and the N2 flow rate and temperature as sheath gas was 11 L/min and 379 °C, respectively. The m/z range 100-1700 in negative ion mode was used. MassHunter B.06.01 software (Agilent Technologies, Santa Clara, CA, USA) was used for all data acquisition. MS data processing was performed using open source software MZmine 2.5232. Quality control was performed throughout the dataset by including blanks, pure standard samples, and control plasma samples. The average RSD for the PFAS in QC samples was 10.6%, showing the robustness of the analytical procedure. The unknown compounds were identified based on exact mass and MS/MS data. |
| Ion Mode: | NEGATIVE |
