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MB Sample ID: SA258363

Local Sample ID:PTEN KO Rep1
Subject ID:SU002673
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:embryonic fibroblasts

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Combined analysis:

Analysis ID AN004237
Analysis type MS
Chromatography type HILIC
Chromatography system Waters Acquity
Column Waters XBridge Amide (100 x 4.6mm,3.5um)
MS Type APCI
MS instrument type QTRAP
MS instrument name ABI Sciex 5500 QTrap
Ion Mode POSITIVE
Units peak area intensity

MS:

MS ID:MS003984
Analysis ID:AN004237
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:QTRAP
MS Type:APCI
MS Comments:Selective reaction monitoring, SRM, of endogenous water-soluble metabolites was performed for steady state analysis. The positive ion mode ESI voltage was +4900. Dwell time was set to 3ms per SRM transition and 1.55 seconds was the total cycle time. Retention time 15-20 seconds. MultiQuant v2.1 software was used to in integrate peak areas for each metabolite SRM transition
Ion Mode:POSITIVE
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