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MB Sample ID: SA271294
| Local Sample ID: | R Control 1A-01_13_1_3229 |
| Subject ID: | SU002808 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004383 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Bruker timsTOF |
| Column | Hamilton Intensity Solo 2 C18(100 x 2.1 mm, 1.8 um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Bruker timsTOF |
| Ion Mode | POSITIVE |
| Units | AU |
MS:
| MS ID: | MS004132 |
| Analysis ID: | AN004383 |
| Instrument Name: | Bruker timsTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | MetaboScape® 4.0 software from Bruker (Bremen, Germany) was used for data processing. The parameters for the detection were as follows: a minimum of 7 spectra were used for peak detection, while for molecular features detection, the minimum intensity threshold was equivalent to 1000 counts. Mass recalibration was performed within a retention time range between 0-0.3 min. Only those features present in at least 2 of 6 samples were considered. Finally, the detected MS/MS spectra assigned onto the bucket table allowed for better viewing and understanding. The main features included retention time, measured m/z, detected fragments, and molecular weight [12]. Data bucketing parameters were as follows: the retention time range was between 0.3 min and 25 min, whereas the mass range began at 50 m/z and finished at 1,300 m/z. A two-tailed independent student’s t-test was utilized to determine the significantly altered metabolites between the two cell lines. The threshold for significance was p-value <0.05. Functional enrichment analysis was constructed utilizing the MetaboAnalyst 5.0 website (https://www.metaboanalyst.ca). |
| Ion Mode: | POSITIVE |
