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MB Sample ID: SA301658
| Local Sample ID: | 137P |
| Subject ID: | SU002918 |
| Subject Type: | Cultured cells |
| Subject Species: | Lentilactobacillus kefiri |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004571 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890B |
| Column | Agilent HP5-MS (30m x 0.25mm, 0.25 um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5977A |
| Ion Mode | POSITIVE |
| Units | normalized intensity |
MS:
| MS ID: | MS004317 |
| Analysis ID: | AN004571 |
| Instrument Name: | Agilent 5977A |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| MS Comments: | The data was analyzed using MS-Dial (version 4.9) for peak detection, deconvolution, alignment, quantification, normalization, and identification. Putative identification of the metabolites was based on the Kovats retention index (RI) and the matching score of the mass spectra with the libraries. Two libraries were used, a local library made from running authentic standards with Kovats RI, and a public spectrum library, the curated Kovats RI with a total of 28,220 compounds (last edited August 21th, 2022, which includes the Fiehn, RIKEN and MoNA databases). The peaks were manually curated reviewed for peak shape, chromatogram alignment integrity and MS/MS match, and the final list of compounds with RI similarities >95% were report-ed. The data was normalized based on the internal standard spiked in the samples during extraction and using LOWESS (locally weighted scatterplot smoothing) for QC-batch normalization. |
| Ion Mode: | POSITIVE |
