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MB Sample ID: SA315424

Local Sample ID:D-Glu/0_2-neg
Subject ID:SU003003
Subject Type:Bacteria
Subject Species:Escherichia coli
Genotype Strain:BL21(DE3)

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Combined analysis:

Analysis ID AN004748 AN004749
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS
Column In-house packed nano column (ID 100 μm, Kinetex 1.7 μm C18 (100 Å) [Phenomenex], 12-15 cm. In-house packed nano column (ID 100 μm, Kinetex 1.7 μm C18 (100 Å) [Phenomenex], 12-15 cm.
MS Type ESI ESI
MS instrument type LTQ-FT LTQ-FT
MS instrument name Thermo Velos Pro Orbitrap Thermo Velos Pro Orbitrap
Ion Mode NEGATIVE POSITIVE
Units Presence/Absence Presence/Absence

MS:

MS ID:MS004494
Analysis ID:AN004748
Instrument Name:Thermo Velos Pro Orbitrap
Instrument Type:LTQ-FT
MS Type:ESI
MS Comments:Top 10 DDA acquisition: Oribitrap Full-Scan at 30k resolving power from 150-2000 m/z. Data-dependent collisional-induced dissociation (CID) fragmentation scans performed with the LTQ ion trap at 30 normalized collision energy with normal scan rate.
Ion Mode:NEGATIVE
Analysis Protocol File:Deuterated_Lipids_Methods_Summary.pdf
  
MS ID:MS004495
Analysis ID:AN004749
Instrument Name:Thermo Velos Pro Orbitrap
Instrument Type:LTQ-FT
MS Type:ESI
MS Comments:Top 10 DDA acquisition: Oribitrap Full-Scan at 30k resolving power from 150-2000 m/z. Data-dependent collisional-induced dissociation (CID) fragmentation scans performed with the LTQ ion trap at 30 normalized collision energy with normal scan rate.
Ion Mode:POSITIVE
Analysis Protocol File:Deuterated_Lipids_Methods_Summary.pdf
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