Return to study ST003077 main page
MB Sample ID: SA332543
| Local Sample ID: | Adipose505 |
| Subject ID: | SU003192 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005035 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Xbridge BEH C18 column (100 × 2.1 mm, 2.5 um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Orbitrap Exploris 480 |
| Ion Mode | UNSPECIFIED |
| Units | arbitrary unit |
MS:
| MS ID: | MS004774 |
| Analysis ID: | AN005035 |
| Instrument Name: | Thermo Orbitrap Exploris 480 |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The relevant parameters of Orbitrap Exploris 480 were as listed: vaporizer temperature, 350 °C; ion transfer tube temperature, 300 °C; sheath gas, 35; auxiliary gas, 7; sweep gas, 1; spray voltage, 3.5 kV for positive mode and 2.5 kV for negative mode; RF-lens (%), 45. The resolution was set at 120,000 (at m/z 200). The scan range was 200 to 1600 (m/z). Automatic maximum injection time (max IT) and automatic gain control (AGC) were used. MS/MS scan was acquired using Hybrid mode (BRI-DIA followed by DDA) with the inclusion list containing precursor ions in positive and negative ion mode (described in the Supplementary table 1 of our prior publication PMID: 35842862). The MS/MS condition for positive or negative ion mode was set as follows: precursor isolation window was set at 1 (m/z) for all three methods, HCD collision energy was set at 25%, orbitrap resolution of full scan and MS/MS scan was set at 60,000 and 15,000, respectively. Intensity threshold for DDA and DIA MS/MS scan was set at 10,000. LC-MS peak extraction and integration were performed using MS-DIAL with default settings. |
| Ion Mode: | UNSPECIFIED |
