Return to study ST003114 main page
MB Sample ID: SA337381
| Local Sample ID: | S16_mitos_linoleic.acid_KO_4 |
| Subject ID: | SU003230 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005102 | AN005103 | AN005104 | AN005105 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | None (Direct infusion) | Normal phase | Reversed phase | Reversed phase |
| Chromatography system | Advion TriVersa NanoMate | Agilent 1260 | Shimadzu Nexera X2 | Shimadzu Nexera X2 |
| Column | None | Macherey-Nagel Nucleosil NH2 (50×2 mm, 3 um, 120 Å) | Waters Acquity BEH Shield RP18 (100×2.1 mm, 1.7 um) | Phenomenex Core-Shell Kinetex Biphenyl (100×3.0 mm, 2.6 um, 100 Å) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | QTRAP | QTRAP | QTRAP | QTRAP |
| MS instrument name | SCIEX QTRAP 6500 | SCIEX QTRAP 6500 | SCIEX QTRAP 6500 | SCIEX QTRAP 6500 |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE | NEGATIVE |
| Units | counts per second (cps) | counts per second (cps) | counts per second (cps) | counts per second (cps) |
MS:
| MS ID: | MS004839 |
| Analysis ID: | AN005102 |
| Instrument Name: | SCIEX QTRAP 6500 |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | PC, PE, PI, PS, PG, and PA species were analyzed by Nano-Electrospray Ionization Tandem Spectrometry (Nano-ESI-MS/MS) with direct infusion of the lipid extract (Shotgun Lipidomics) as previously described (Kumar et al., J Cell Biol 2015, 211, 1057). |
| Ion Mode: | POSITIVE |
| MS ID: | MS004840 |
| Analysis ID: | AN005103 |
| Instrument Name: | SCIEX QTRAP 6500 |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | LC-ESI-MS/MS analysis of ceramides and sphingomyelins was conducted as previously published (Oteng et al., J Lipid Res 2017, 58, 1100; Schwamb et al., Blood 2012, 120, 3978). |
| Ion Mode: | POSITIVE |
| MS ID: | MS004841 |
| Analysis ID: | AN005104 |
| Instrument Name: | SCIEX QTRAP 6500 |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Cardiolipin (CL) species were analyzed by Liquid Chromatography coupled to Electrospray Ionization Tandem Mass Spectrometry (LC-ESI-MS/MS). CL species were monitored in the positive ion mode using the following Multiple Reaction Monitoring (MRM) transitions: CL 68:4, m/z 1418.9 to 575.4; CL 70:4, m/z 1446.9 to 575.4; CL 72:4 m/z 1475.0 to 603.4; CL 61:1 (internal standard), m/z 1326.9 to 535.4. For all MRM transitions the values for declustering potential, entrance potential, collision energy, and cell exit potential were 140 V, 10 V, 45 V, and 7 V, respectively (Tatsuta, Methods Mol Biol 2017, 1567, 79). The instrument settings for nebulizer gas (Gas 1), turbo gas (Gas 2), curtain gas, and collision gas were 50 psi, 50 psi, 40 psi, and medium, respectively. The Turbo V ESI source temperature was 500 °C, and the ionspray voltage was 4.5 kV. The LC chromatogram peaks of the endogenous CL species and the internal standard CL 61:1 were integrated using the MultiQuant 3.0.2 software (SCIEX). |
| Ion Mode: | POSITIVE |
| MS ID: | MS004842 |
| Analysis ID: | AN005105 |
| Instrument Name: | SCIEX QTRAP 6500 |
| Instrument Type: | QTRAP |
| MS Type: | ESI |
| MS Comments: | Fatty acid levels were determined by LC-ESI-MS/MS: Fatty acids were monitored in the negative ion mode using “pseudo” Multiple Reaction Monitoring (MRM) transitions (Hellmuth et al., Anal Chem 2012, 84, 1483). The instrument settings for nebulizer gas (Gas 1), turbo gas (Gas 2), curtain gas, and collision gas were 60 psi, 90 psi, 40 psi, and medium, respectively. The Turbo V ESI source temperature was 650 °C, and the ionspray voltage was -4 kV. The LC chromatogram peaks of the endogenous fatty acids and the internal standard palmitic-d31 acid were integrated using the MultiQuant 3.0.2 software (SCIEX). |
| Ion Mode: | NEGATIVE |
