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MB Sample ID: SA338919
| Local Sample ID: | H_WTCD_9 |
| Subject ID: | SU003243 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005125 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | Merck SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | Peak Intensity |
MS:
| MS ID: | MS004861 |
| Analysis ID: | AN005125 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The MS data was processed using XCalibur 4.1 (Thermo Scientific) to obtain the metabolite signal intensity for relative quantitation. Targeted identification was available for 205 metabolites based on an in-house library established using known chemical standards. Identification required exact mass (within 5ppm) and standard retention times. For untargeted metabolomics, metabolites were identified by mass matching of the MS signal to metabolites in the HMDB database. If multiple metabolites in the database were matched to a certain MS signal, all matched metabolites were grouped into a single identification, and ordered based on the number of references included in the HMDB database (high to low). We used the first ranked metabolite in the downstream analyses. When multiple values with the same metabolite attribution occurred (different metabolites with same mass and retention time), we opted to use all values in the analyses to avoid biases on which intensities/attributions to consider. Peak intensities for metabolites were screened for missing values and relative metabolite abundance data was analyzed by using MetaboAnalyst software version 5.0 (Pang et al, 2021). Metabolite significance was determined with one-way ANOVA with post-hoc t-tests, with the cutoff being a raw p value < 0.05, and the pathway significance cutoff was FDR corrected p value < 0.05. |
| Ion Mode: | UNSPECIFIED |
| Analysis Protocol File: | Protocol_HeartMetabolomics.pdf |
