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MB Sample ID: SA340621

Local Sample ID:Sample7
Subject ID:SU003259
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

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Combined analysis:

Analysis ID AN005155 AN005156
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Bruker Impact HD Bruker Impact HD
Ion Mode POSITIVE NEGATIVE
Units peak area peak area

MS:

MS ID:MS004891
Analysis ID:AN005155
Instrument Name:Bruker Impact HD
Instrument Type:QTOF
MS Type:ESI
MS Comments:The acquisition rate was 1.44 Hz for MS acquisition and 4 – 10 Hz for MS/MS spectra acquisition, with an m/z range from 150 to 1500. For both positive and negative ionization. Intensity threshold:2500 cts for positive ionization. Lipid features were extracted and aligned using software LipidScreener 1.1.0
Ion Mode:POSITIVE
Capillary Voltage:4200 V
Collision Energy:10-70 eV
Dry Gas Flow:5.0 L/min
Dry Gas Temp:240°C
Nebulizer:1.2 Bar
  
MS ID:MS004892
Analysis ID:AN005156
Instrument Name:Bruker Impact HD
Instrument Type:QTOF
MS Type:ESI
MS Comments:The acquisition rate was 1.44 Hz for MS acquisition and 4 – 10 Hz for MS/MS spectra acquisition, with an m/z range from 150 to 1500. For both positive and negative ionization. Intensity threshold:1500 cts for negative ionization. Lipid features were extracted and aligned using software LipidScreener 1.1.0
Ion Mode:NEGATIVE
Capillary Voltage:4200 V
Collision Energy:10-70 eV
Dry Gas Flow:5.0 L/min
Dry Gas Temp:240°C
Nebulizer:1.2 Bar
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