Summary of Study ST001952
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001239. The data can be accessed directly via it's Project DOI: 10.21228/M8JT51 This work is supported by NIH grant, U2C- DK119886.
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Study ID | ST001952 |
Study Title | GLS2KO vs WT mouse hepatocytes |
Study Type | Genotype |
Study Summary | Test the effect of GLS2 knockout in primary mouse hepatocytes. We isolated hepatocytes from GLS2 knockout and wild-type mice, and briefly applied media lacking L-glutamine (2 hours). Sixty minutes after resupplying Gln, metabolites were extracted and analyzed with the Mixed Mode method. Data were processed through XCMS, and features were filtered for p<0.01, fold change >2, and a minimum intensity of 1x10^6. Sorting by smallest p value, the first extracted ion chromatogram (EIC) with good chromatographic peak shape corresponded to 188.0567m/z at 24.41 min. Six putative IDs were within 3ppm of the experimentally observed m/z, representing two chemical formulas, none of which had documented retention times in training or test sets. Amongst these potential IDs, the Message Passing Neural Network (MPNN) model correctly predicted N-acetyl-L-glutamic acid as the most likely candidate, as verified by injection of purchased standards. The next most significant difference between GLS2KO vs WT was 117.0196m/z observed at 20.07 minutes. The model had been trained on 2/6 of the putative IDs. Despite the four additional isomers suggested, the model correctly selected succinate as reduced by GLS2 KO (Figure 5B). The third most significant hit corresponds to 171.0068m/z at 23.11 min. Although glycerol 1-P and 2-P are both potential hits, almost indistinguishable by the model, the large gap in retention times between these top hits and the Cl- adducts of threonate (+ isomers) is apparent, further supporting the correct identification as glycerol mono-phosphate. Expansion of the list to include p<0.05 leads to the identification of Glutamine, Glutamate, and other downstream metabolites known to be altered by GLS2 KO. |
Institute | Pfizer |
Last Name | Clasquin |
First Name | Michelle |
Address | 1 Portland St., Cambridge, MA 02139 |
michelle.clasquin@pfizer.com | |
Phone | 6174487289 |
Submit Date | 2021-10-22 |
Num Groups | 2 |
Total Subjects | 6 |
Num Males | 6 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2021-11-08 |
Release Version | 1 |
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Combined analysis:
Analysis ID | AN003177 |
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Analysis type | MS |
Chromatography type | Unspecified |
Chromatography system | Dionex UltiMate 3000 RSLC |
Column | HILICpak VT50 2D (150 mm x 2.0 mm,5um particle size,Shodex,Japan) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap |
Ion Mode | NEGATIVE |
Units | Peak area |
MS:
MS ID: | MS002955 |
Analysis ID: | AN003177 |
Instrument Name: | Thermo Q Exactive Plus Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | The Mass Spec parameters are set as Source Fragmentation: None; Sheath gas flow rate: 45; Aux gas flow rate: 15; Sweep gas flow rate: 3; Spray voltage: 3.00 kV; Ca-pillary temp: 310°C; S-lens RF level: 50; Aux gas heater temp: 350°C; For Full MS: Scan range: 65.0 to 975.0 m/z; Resolution: 140,000; Polarity: Negative; AGC target: 3e6; Max-imum IT: 500 ms. |
Ion Mode: | NEGATIVE |