Summary of Study ST001776
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001130. The data can be accessed directly via it's Project DOI: 10.21228/M8N706 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001776 |
| Study Title | Metabolic Response of HEK 293 Cells Exposed to Methylmercury |
| Study Summary | HEK 293 cells were treated with 7.5 μM methylmercury (HgMe) for 48 h. Metabolites were extracted and subjected to LC-HRMS based metabolomics. LC-HRMS data were processed by SIEVE2.2 software. Metabolites associated with HgMe toxicity were screened and identified. |
| Institute | University of Macau |
| Last Name | Zhang |
| First Name | pw |
| Address | Taipa, Macau SAR, China |
| yb47620@um.edu.mo | |
| Phone | 8613924251358 |
| Submit Date | 2021-04-27 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-01-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002883 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 RS |
| Column | Thermo Accucore HILIC (100 x 2.1mm,2.6um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | arbitray unit |
MS:
| MS ID: | MS002676 |
| Analysis ID: | AN002883 |
| Instrument Name: | Thermo Q Exactive Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | The MS acquisition parameters were as follows: spray voltage, 3.5 kV; capillary temperature, 320 °C; sheath gas flow rate, 45; auxiliary gas flow rate, 25; heater temperature 350 °C; AGC, 3×106; maximum injection time, 200 ms; mass scan range, 50–750; full MS resolution, 70,000 FWHM at m/z 200; spectrum data type, profile. The raw LC-HRMS files for the same study were processed in one batch using the label-free differential analysis software (SIEVE 2.2, Thermo Scientific), in which the ChromAlign algorithm was used. The key parameters for the feature extraction were as follows: signal to background noise,>3; Mzstep accuracy, 10 ppm, minimum peak intensity 200,000; minimum peak scan points, 5; minimum isotopes,1. |
| Ion Mode: | POSITIVE |
