Summary of Study ST002699
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001671. The data can be accessed directly via it's Project DOI: 10.21228/M8Q716 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002699 |
| Study Title | Spatially resolved metabolomics and isotope tracing reveal dynamic metabolic responses of dentate granule neurons with acute stimulation |
| Study Summary | KCl timecourse|Neuronal activity creates an intense energy demand that must be met by rapid metabolic responses. To define the metabolic adaptations of the neuron-enriched dentate granule cell (DGC) layer within the native tissue environment, we utilize acute hippocampal brain slices and perform fast metabolite preservation followed by mass spectrometry imaging (MALDI-MSI) to obtain spatially resolved metabolomics and isotope tracing data. We find that membrane depolarization promotes broad metabolic changes, including increased glycolytic activity in DGCs which is accompanied by mobilization of endogenous inosine into pentose phosphates. |
| Institute | Harvard University |
| Last Name | Yellen |
| First Name | Gary |
| Address | 220 Longwood Ave |
| gary_yellen@hms.harvard.edu | |
| Phone | 617-432-0137 |
| Submit Date | 2023-04-04 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | imzML |
| Analysis Type Detail | MALDI-MS |
| Release Date | 2023-08-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004374 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | none |
| Column | none |
| MS Type | MALDI |
| MS instrument type | QTOF |
| MS instrument name | Bruker timsTOF fleX |
| Ion Mode | NEGATIVE |
| Units | a.u. |
MS:
| MS ID: | MS004123 |
| Analysis ID: | AN004374 |
| Instrument Name: | Bruker timsTOF fleX |
| Instrument Type: | QTOF |
| MS Type: | MALDI |
| MS Comments: | Mass spectra were acquired on a timsTOF fleX in negative ion mode. Hippocampal sections were outlined as individual ROIs with a pixel size of 20 µm. Most metabolites were acquired in a single imaging run (m/z range 50 - 1000). Each MSI pixel consisted of 1000 laser shots. The laser Smartbeam parameter was set to single with a frequency of 10 kHz. |
| Ion Mode: | NEGATIVE |
