Summary of Study ST003007
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001873. The data can be accessed directly via it's Project DOI: 10.21228/M8MF0Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003007 |
Study Title | 15N BCAA tracing in brown adipocyte |
Study Summary | To determine the metabolic fate and nitrogen flux of BCAA in mouse brown adipocytes, we used 15N labeled BCAA tracing (Leu (NLM-142-1, CIL), Ile (NLM-292-0.25, CIL) and Val (NLM-316-0.5, CIL)) followed by LC-MS analysis. |
Institute | Harvard Medical School |
Last Name | Wang |
First Name | Dandan |
Address | 3 Blackfan Circle |
dandanwang2022@gmail.com | |
Phone | 5083733714 |
Submit Date | 2023-12-13 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2024-02-19 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004938 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Vanquish Horizon |
Column | Waters ACQUITY UPLC BEH Amide (100 × 2.1mm, 1.7um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo orbitrap exploris 240 |
Ion Mode | NEGATIVE |
Units | Peak area |
MS:
MS ID: | MS004681 |
Analysis ID: | AN004938 |
Instrument Name: | Thermo orbitrap exploris 240 |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | ESI source parameters were set as follows: spray voltage, 3500 V or −2800 V, in positive or negative modes, respectively; vaporizer temperature, 350 °C; sheath gas, 50 arb; aux gas, 10 arb; ion transfer tube temperature, 325 °C. The full scan was set as: orbitrap resolution, 60,000; maximum injection time, 100 ms; scan range, 70–1050 Da. The ddMS2 scan was set as: orbitrap resolution, 30,000; maximum injection time, 60 ms; top N setting, 6; isolation width, 1.0 m/z; HCD collision energy (%), 30; Dynamic exclusion mode was set as auto. The metabolites was quantified by Compound Discoverer 3.3. |
Ion Mode: | NEGATIVE |