Summary of Study ST000134
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000119. The data can be accessed directly via it's Project DOI: 10.21228/M86C76 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000134 |
Study Title | Monitoring In Vitro Response of Selenium-Treated Prostate Cells by 1H NMR Spectroscopy |
Study Type | Metabolite level response after treatment with organoselenium |
Study Summary | Metabolomics analysis was performed on DU145 prostate cancer cells and PNT1A non-tumorigenic prostate cells after treatment with selenomethionine and Se-methylselenocysteine using 800 MHz Bruker NMR spectrometer on 18 cell samples. |
Institute | Purdue University |
Department | Biology/Chemistry |
Laboratory | Biology/Chemistry |
Last Name | Isaac-Lam |
First Name | Meden |
Address | 1401 S US Hwy 421, Westville, IN 46391 |
isaaclam@pnc.edu | |
Phone | 219-785-5776 |
Submit Date | 2015-01-13 |
Num Groups | 6 |
Total Subjects | 18 |
Raw Data Available | No |
Analysis Type Detail | NMR |
Release Date | 2015-01-13 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
NMR:
NMR ID: | NM000050 |
Analysis ID: | AN000216 |
Instrument Name: | Bruker Avance III |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Field Frequency Lock: | Deuterium |
Standard Concentration: | 1.43 mM |
Spectrometer Frequency: | 800 MHz |
NMR Probe: | TX1, inverse |
NMR Solvent: | D2O |
NMR Tube Size: | 5 mm x 7 in |
Shimming Method: | gradient, topshim |
Pulse Sequence: | zg one-pulse |
Water Suppression: | presaturation |
Pulse Width: | 8.5 msec |
Power Level: | 12.25 W |
Receiver Gain: | 912 |
Offset Frequency: | 4.8 ppm |
Presaturation Power Level: | 57 dB |
Chemical Shift Ref Cpd: | TMSP |
Temperature: | 295.9 |
Number Of Scans: | 256 |
Dummy Scans: | 8 |
Acquisition Time: | 1.4680564 |
Relaxation Delay: | 6.5 msec |
Spectral Width: | 11160 Hz |
Num Data Points Acquired: | 32 K |
Real Data Points: | 32 K |
Line Broadening: | 1 Hz |
Zero Filling: | 16K |
Apodization: | lorentzian |
Baseline Correction Method: | polynomial |
Chemical Shift Ref Std: | TMSP |